血小板衍生膜微粒对鸡胚绒毛尿囊膜血管新生的影响  

Effects of Platelet-derived Membrane Microparticles on Angioge-nesis in Chick Chorioallantoic Membranes

作　　者：陈宝安[1] 仲悦娇[1] 黄成垠[2] 李翠萍[2] 史光耀[2] 肖建宇[2] 唐荣才[2] 丁家华[1] 高冲[1] 孙耘玉[1] 程坚[1] 王骏[1] 赵刚[1] 马燕[1] 宋慧慧[1] 费菲[1] 裴孝平[1] 

机构地区：[1]东南大学中大医院血液科,南京210009 [2]江苏省血液中心,南京210042

出　　处：《中国实验血液学杂志》2007年第5期1070-1073,共4页Journal of Experimental Hematology

基　　金：江苏省卫生厅科研基金资助课题;编号H200533

摘　　要：本研究探讨血小板衍生膜微粒(platelet-derived membrane microparticles,PMP)对鸡胚绒毛尿囊膜(CAM)新生血管形成的影响。用凝血酶激活血小板释放出PMP,再经高速离心分离出PMP,用流式细胞仪检定PMP,并用BCA法测定PMP的含量。将PMP接种于鸡胚绒毛尿囊膜上,观察PMP对CAM血管生成的影响。结果显示:当PMP的浓度为80μg/ml时,孵育72小时后混合纤维素滤膜周围可见到放射状密集生长的血管网,血管分支数为112.5±11.31,血管面积为(6.19±1.29)(,而在对照组无特异性密集血管生成,血管分支数为82.6±8.05,血管面积为1.78±0.33,二组比较有显著性差异(P<0.05);而与VEGF组比较,后者的血管分支数为128.4±10.02,血管面积为7.44±1.36,二组比较差异无显著性。结论:PMP对CAM新生血管的生成有促进作用。This study was purposed to investigate the angiogenesis effect of platelet-derived membrane microparticles （PMPs） in chick chorioallantoic membranes （CAM） . Thrombin were adopted to activate the platelets and then PMPs were obtained. PMPs were isolated by high speed centrifugation. Flow cytometry （FCM） was adopted to evaluate the efficiency of thrombin to produce PMPs and BCA method was adopted to evaluate the content of PMPs. PMPs were put into CAM and the effects of PMPs on angiogenesis in CAM were observed. The results indicated that after incubation for 72 hours at the concentration of 80 μg/ml PMPs, the vessel nets in a ＇ spoked-wheel＇ pattern were shown around mixed fibrous filter membranes, number of vessel ramification was 112, 5 ± 11.31 and ratio of vessel area/CAM area was 6.19 ± 1.29 % , but there were not localized allantoic vessels developing in the control group, the number of vessel ramification and ratio of vessel area/CAM area in control group were 82.6 ± 8.05 and 1.78 ± 0.33 respectively, so there was significant difference between PMP and control groups. In above mentioned conditions, the number of vessel ramification and ratio of vessel area/CAM area in VEGF group were 128.4 ± 10.02 and 7. 44 ± 1,36 respectively, so there was no difference between PMP and VEGF groups. It is concluded that PMPs show promotive effect on the formation of capillaries in chick chorioallantoic membranes.

关 键 词：血小板膜衍生微粒 鸡胚绒毛尿囊膜 血管生成 

分 类 号：R331.145[医药卫生—人体生理学]