修复基因XRCC1、XPD和XRCC3多态性与苯致DNA损伤修复能力关系的研究  

Association between genetic polymorphisms of DNA repair genes XRCC1,XPD,XRCC3 and the capacity of DNA repair induce by benzene

作　　者：许建宁[1] 杨敏[1] 黄慧隆[1] 王全凯[1] 王雅文[1] 李桂兰[1] 

机构地区：[1]中国疾病预防控制中心职业卫生与中毒控制所,北京100050

出　　处：《卫生研究》2007年第5期529-532,共4页Journal of Hygiene Research

基　　金：国家自然科学基金资助项目(No.30300279)

摘　　要：目的探讨XRCC1、XPD、XRCC3基因多态性与苯致DNA损伤修复能力的关系。方法以确诊并已脱离苯作业的80名慢性苯中毒患者作为病例组,以同期接苯的62名苯作业工人为对照组,应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析技术,检测XRCC1 C26304T(Arg194Trp)、G27466A(Arg280His)、G28152A(Arg399Gln)、G36189A(Gln632Gln)和XPD C22541A(Arg156Arg)、C23591T(Asp312Asn)、A35931C(Lys751Gln)以及XRCC3 C18067T(Thr241Met)位点的多态性,采用细胞阻滞微核试验和碱性彗星试验分别从细胞水平和分子水平检测DNA修复能力。结果携带XPD35931 AC+CC变异基因型的个体、携带XPCC318067C/T变异基因型的个体均比携带相应野生基因型个体的苯致DNA损伤修复能力强。Objective To explore the correlation between genetic polymorphisms of XRCC 1, XPD, XRCC3 and DNA repair capacity induced by benzene. Methods Eighty patients suffered from chronic benzene poisoning were investigated. PCR-RFLP was applied to detect the single nucleotide polymorphisms on C26304T, G27466A, G28152A, G36.189A of XRCC1, C22541A, C23591T, A35931C of XPD, C18067T of XRCC3. Cytokinesis-block micronucleus （CBMN） and alkaline comet were applied to detect the DNA repair capacity. Results The DNA repair capacity of the subjects carrying XPD 35931C variant alleleor carrying XRCC3 18067 C/T variant genotype were higher than those carrying corresponding mild genotype. Conclusion There could be a correlation between polymorphisms of XRCC3 and DNA repair capacity of DNA damage induced by benzene.

关 键 词：XRCC1 XPD XRCC3 基因多态性 苯中毒 DNA修复能力 

分 类 号：R135.12[医药卫生—劳动卫生] Q523[医药卫生—公共卫生与预防医学]