乙醇对体外培养小鼠中脑细胞发育的影响研究  

Effect of ethanol on the development of mouse neuroblasts

作　　者：许雅君[1] 李勇[1] 

机构地区：[1]北京大学公共卫生学院营养与食品卫生学系,北京100083

出　　处：《卫生研究》2007年第5期568-571,共4页Journal of Hygiene Research

基　　金：国家自然科学基金资助项目(No.30271364)

摘　　要：目的探讨酒精对胚胎中脑细胞发育的影响及其与畸形发生之间的联系。方法从12天龄、体节数34～36的小鼠胚胎分离中脑神经母细胞接种培养板,给予不同剂量的酒精于体外培养3d或5d后,检测酒精对细胞分化、神经纤维蛋白表达和凋亡的影响。结果酒精对中脑神经母细胞分化有抑制作用,随酒精染毒剂量的增加,神经纤维蛋白表达下调,细胞凋亡比例逐渐增加,存在剂量-反应关系。结论酒精通过抑制发育期神经母细胞的分化、抑制神经纤维蛋白表达以及诱导细胞过度凋亡而导致中枢神经系统畸形。Objective To investigate the effect of ethanol on the development of embryonic neuroblasts and the relation to ethanol＇s teratogenicity. Methods 12 day mouse embryos with 34- 36 somites were separated from the dam. The embryonic midbrain was cut off and neuroblasts were prepared for primary culture. The neuroblasts were cultured with different concentrations of ethanol. After 3 - 5 days, the cell differentiation, neurofilament protein level and apoptosis were detected. Results Differentiation of the midbrain neuroblasts were inhibited by ethanol exposure. As the ethanol concentration increasing, the level of neurofilament protein decreased and the number of cells undergoing apoptosis increased, showing a dose dependant style. Conclusion Alcohol could inhibit the differentiation of midbrain neuroblasts, suppressed the express of neurofilament protein and induce excessive apoptosis, which may be related to the teratogenic action of ethanol on central nervous system.

关 键 词：酒精 细胞分化 神经纤维蛋白 凋亡 致畸性 中脑细胞 

分 类 号：Q132.7[生物学—普通生物学] Q593.2