CD14的B细胞抗原表位预测  

Prediction of B-cell antigen epitope on CD14.

作　　者：冯起甲[1] 徐智[1] 吴国明[1] 胡川闽[2] 徐顺贵[1] 李树钧[1] 钱桂生[1] 

机构地区：[1]第三军医大学新桥医院全军呼吸内科研究所全军呼吸病研究重点实验室,重庆400037 [2]第三军医大学医学检验系临床生物化学教研室,重庆400038

出　　处：《四川医学》2007年第9期959-961,共3页Sichuan Medical Journal

基　　金：国家自然科学基金(30500230)

摘　　要：目的预测人CD14抗原的B细胞表位。方法采用生物信息软件和互联网服务器，预测CD14的二级结构，分析CD14表面特性（亲水性、可塑性、可及性和抗原性），再计算平均抗原指数（AI）预测CD14的B细胞抗原表位。结果CD14存在多个潜在的抗原表位，257-271序列（shnslratvnpsapr）的AI（0.04413）明显高于其它序列，305-321序列（sc-nrlnrapqpdelpev）、19-34序列（ttpepcelddedfrc）和115-134序列（ysrlkeltledlkitgtmpp）的AI分别是0.03682、0.03256和0.03025。结论257-271序列（shnslratvnpsapr）是CD14的B细胞优势抗原表位，可用于制备CD14的特异性抗体。Objective To predict the B cell epitope for lipepolysaccharide- binding protein（CD14） antigen.Methods By using biotic softwares and network servers,the secondary structure of CD14 was predicted,and the surface properties of CD14,such as hydrophilicity,flexibility, accessibility and antigenicity were analysed. Then the average antigen index（AI） was calculated by using Wu＇s method, which could predict the antigen epitope of CD14. Results Many distinct antigenic epitopes on CD14 were identified by computation：The AI of sequence 257 - 271 （shnslratvnpsapr）on CD14 was 0.04413, which was significantly higher than that of other sequences. The AI of sequence 305 - 321 （Scnrlnrapqpdelpev ）, sequence 19 - 34（ttpepcelddedfrc ） and sequence 115 - 134 （ysdkeltledlkitgtmpp ） were 0.03682,0.03256 and 0.03025, respectively. Conclusion Sequence 257 - 271 （ shnslratvnpsapr ） is the prevalent antigen epitope of CD14,and is used for preparing specific antibody of CD14.

关 键 词：CD14 抗原 表位 B细胞 生物信息学 

分 类 号：R392.1[医药卫生—免疫学]