球孢白僵菌不同菌株生物学特性及对小菜蛾的毒力研究  

Study on the Biological Characters of Isolates of Beauveria bassiana and their Virulence Against the Plutella xylostella

作　　者：孙鹏飞[1] 陈斌[1] 李正跃[1] 和淑琪[1] 

机构地区：[1]云南农业大学植物保护学院,云南昆明650201

出　　处：《云南农业大学学报》2007年第5期635-638,644,共5页Journal of Yunnan Agricultural University

基　　金：云南省科技重点攻关项目(2100NG57);云南省科技厅项目(2003C0017Q);云南省教育厅(02QY053)

摘　　要：室内测定了从鳞翅目小菜蛾、斜纹夜蛾和块茎蛾罹病虫体上分离纯化的球孢白僵菌菌株DBM001，XW-YE001和PTM001的菌落生长速率、产孢量、孢子萌发速度，及对小菜蛾幼虫的致病力，并用时间-剂量-死亡率模型进行了分析。结果表明，各菌株在菌落生长速率上无显著差异，但各菌株的孢子萌发速率有显著差异。供试DBM001，PTM001 XWYE001三菌株对小菜蛾二龄幼虫的毒力测定结果表明：供试3菌株对于小菜蛾均具有一定的毒力，剂量效应参数分别为0．424，0．304，0．297。在10^8孢子／mL，10^7孢子／mL，10^6孢子／mL，10^5孢子／mL，10^4孢子／mL的孢子悬浮液下，3菌株孢子液处理后小菜蛾的累积死亡率分别为94．0％～48．0％，76．0％～28．0％和62．0％～28．0％。3菌株处理后第3d时的LC50分别为6．5×10^7孢子／mL；4．07×10^7孢子／mL和2．88×10^7孢子／mL。在10^6孢子／mL下，3菌株对小菜蛾幼虫的致死亡中时LT50依次为3．0，4．1，3．5d。由此表明，斜纹夜蛾和马铃薯块茎蛾菌株的对小菜蛾幼虫的致死时间效应较长，均在3d以上，即球孢白僵菌不同菌株对小菜蛾种群增长的抑制作用时效差异较大，尤其是斜纹夜蛾和马铃薯块茎蛾分离株对小菜蛾的毒力发挥较慢。The biolgical characters of three isolates of Beauveria bassiana was tested, isolates were marked as DBM001, XWYE001 and PTM001. More over, the virulence against the diamondback moth, Plutella xylostella was evaluated. Among the tested isolates, no significant difference was found in spore yield, germination rate, as well as the mycelium growth rates on SDAY medium. But the virulence against diamondback moth was similar. A time-dose-mortality model was used for analyzing the mortality as affected by both time and dosage. The results showed that all tested isolates were virulent to the larvae of diamondback moth, accumulative mortality was 94. 0%- 48.0% ,76. 0% - 28.0%, 62.0% -28.0%. The value of LC50 for the these three isolate was 6. 5 × 10^7conidia/mL;4. 07 ×10^7conidia/mL;2.88 × 10^7conidia/mL, on the 3rd day after inoculation, the LT50 for the 3 isolates ranged from 3.0, 3.5, 4. 1 days under the inoculation of 10^6 conidia/mL.

关 键 词：球孢白僵菌 生物学特性 生物测定 毒力 小菜蛾 

分 类 号：Q935[生物学—微生物学]