云南甘蔗宿根矮化病病原检测  

Primary Pathogen Detection of Sugarcane Ratoon Stunting Disease in Yunnan

作　　者：黄应昆 李文凤[1] 赵俊[1] 饶云 卢文洁[1] 李俊[1] 罗志明[1] 杨洪昌[1] 

机构地区：[1]云南省农业科学院甘蔗研究所,云南开远661600 [2]云南省新平县农业局,云南新平653400

出　　处：《云南农业大学学报》2007年第5期762-765,共4页Journal of Yunnan Agricultural University

基　　金：国家"948"项目(2003-Q06);云南省重大农业推广项目(2005)

摘　　要：利用从澳大利亚引进的甘蔗宿根矮化病病原(RSD)的标准抗原抗体,建立了I-ELISA检测甘蔗宿根矮化病的方法。采用电镜负染法和I-ELISA法,对采自云南红河、开远、新平蔗区的42个样本进行RSD检测,结果表明:29个样本为阳性,13个样本为阴性,确认云南蔗区存在RSD。The mean of I - ELISA of detecting pathogen of RSD is established based on standard antigen and antibody from Australia. 42 samples from Honghe, Kaiyuan and Xinping were detected by means of negative staining of electron microscope and I - ELISA. The results indicated that 29 samples are positive and 13 samples are negative, so as to prove that RSD occur in Yunnan.

关 键 词：云南 甘蔗宿根矮化病 发生 检测 

分 类 号：S435.661[农业科学—农业昆虫与害虫防治]