Wistar大鼠感染痘病毒观察  

Observation on the Natural Poxvirus Infecton in a Colony of Wistar Rat

作　　者：李嘉荣[1,2] 钱琴 叶乃青[1,2] 宋新英 屈雅琴 陈天培 

机构地区：[1]卫生部上海生物制品研究所医学实验动物中心 [2]上海第二医科大学附属仁济医院

出　　处：《上海实验动物科学》1997年第1期3-5,29,共4页Shanghai Laboratory Animal Science

摘　　要：３０只无合格证的Ｗｉｓｔａｒ大鼠发生了酷似小鼠脱脚病的疾病，发病率高达３３．３％（１０／３０）。取病鼠的皮肤经组织学检查，发现其病理变化呈痘病毒感染的特征性改变。病鼠的肝、脾匀浆接种于ＳＰＦ鸡胚绒毛尿囊膜后，出现痘斑并已连续传至第４代，经电镜检查发现有成熟的病毒粒子和许多幼稚型病毒。病毒形态与痘病毒一致，有囊膜，直径在１７０～２１２．５ｎｍ之间。异常肝、脾组织和经传代接种的鸡胚绒毛尿囊膜的匀浆上清液，对鸡红细胞均呈阳性凝集反应；但接种于豚鼠角膜呈阴性反应。用小鼠痘病毒ＥＬＩＳＡ试剂盒检测病鼠血清呈阴性反应。An outbreak of poxvirus infection was occurred in a Wistar rat colony,with a morbidity of 33.3%(10/30). The clinical signs and the pathohistological findings of the tail skin, liver and spleen lesions were identical with those of mouse pox. The DNA of the virus was found in the epithelial cytoplasm by using Feulgen stain. Suspension of the homogenized liver and spleen tissues were inoculated onto chorioallantoic membranes of 12day chick embryos. Pocks were appeared on the membranes at the fourth passage. Electron microscopic examination revealed the presence of poxvirus in both the chorioallantoic membranes and the suspension of the homogenized tissues.

关 键 词：大鼠 痘病毒感染 自发感染 

分 类 号：S858.953[农业科学—临床兽医学]