基因芯片对荷瘤裸鼠Tca8113/卡铂耐药基因表达谱的分析  

Analysis of gene expression profiles in Tca8113/CBP nude mice by microarray technique

作　　者：冯戈[1] 王大章[1] 陈槐卿[2] 何嘉[1] 冷卫东[3] 

机构地区：[1]四川大学教育部口腔生物医学工程重点实验室,610041 [2]四川大学生物医学工程研究中心(博士后流动站) [3]湖北十堰市郧阳医学院附属太和医院口腔中心

出　　处：《实用口腔医学杂志》2007年第5期688-691,共4页Journal of Practical Stomatology

基　　金：国家自然科学基金资助项目(编号:39970798);四川大学优秀博士论文研究基金资助项目(编号:0040305505010)

摘　　要：目的:研究人舌鳞癌肿瘤组织和耐药组织中的基因表达差异,探讨肿瘤耐药的作用机制,为临床化疗提供实验依据。方法:BALB/c-nu/nu裸鼠皮下接种Tca8113细胞悬液,待成瘤后随机分为化疗诱导组(Tca8113/CBP)15只和对照组(Tca8113)5只。诱导组采用卡铂(CBP)进行小剂量连续诱导10周,将Tca8113组和Tca8113/CBP组肿瘤组织,用人16k cDNAv2.1SBC-R-HC-100-21基因芯片对其基因表达谱进行聚类分析。结果:舌鳞癌Tca8113荷瘤裸鼠和Tca8113/卡铂(CBP)诱发瘤裸鼠中表达差异基因上调基因435条,下调基因284条。结论:卡铂的诱导化疗使一组基因在舌鳞癌中表达改变,为筛选与舌鳞癌耐药发生有关的基因群,进一步深入研究其中某些关键基因提供导向性资料。Objective：To study the differences of gene expression between Tca8113 and Tca8113/CBP tissues in nude mice. Methods：Tca8113 cells were injected subcutaneously in both sides of armpits of nude mice at the concentration of 5 × 10^6 cells/0.1 ml. Two weeks after injection, Carboplatin was used subcutaneously around the tumor 0.01 mg/g （weight） each day in TcaS113/CBP group while Tca8113 group was injected with physiologic saline as control. Mice were sacrificed 10 weeks after drug injection. The two kinds of tissues were investigated by human 16k cDNA v2.1 SBC-R-HC-100-21 gene chip. Results：Among the 16 000 target genes, there were 719 genes whose expression levels showed differences between the two kinds of tumor tissues. Conclusion： Microarray technique can simultaneously screen different genes from above-mentioned two kinds of tissues. Further analysis of the obtained genes will be helpful to understand the molecular mechanism of muhidrug resistance.

关 键 词：TCA8113细胞 卡铂 多药耐药 基因芯片 

分 类 号：Q789[生物学—分子生物学]