高效降解PCBs的优势菌种选育研究  

Breeding of dominant strains for high-effective degradation of polychlorinated biphenyls

作　　者：李爽[1] 张兰英[1] 赵喆[1] 马会强[1] 宗芳[1] 马莉[1] 

机构地区：[1]吉林大学环境与资源学院,吉林长春130026

出　　处：《水资源保护》2007年第5期47-49,54,共4页Water Resources Protection

基　　金：国家"973"项目(2004CB418505)

摘　　要：以PCBs为目标污染物,筛选出具有高效降解能力的优势菌株P1和P2,通过16SrDNA序列的测定和同源性比较分析,分别将其鉴定为产碱菌属(Alcaligenes sp.)和芽孢杆菌属(Bacillus sp.),并通过驯化提高其降解能力。通过实验研究生长条件对PCBs去除效果的影响。实验得出最佳降解条件:温度为30℃,pH值为7,菌体接种量为5 mL。7 d的降解率最高可达98.2%。Through isolating and purifying,the dominant strains P1 and P2 for high-effective degradation of PCBs were successfully identified as Alcaligenes sp. and Bacillus sp.respectively according to the measurement and homology analysis of their 16SrDNA sequences.By acclimation,their degradation capabilities were enhanced.The effects of growth conditions on the removal efficiency of PCBs were studied by experiment,and the best conditions were 30℃,pH=7,and 5mL of inoculation amount.The degradation ratio could reach 98.2% after 7 days.

关 键 词：PCBS 菌种 选育 生物降解 降解特性 

分 类 号：X703[环境科学与工程—环境工程]