荧光光谱法研究利福平与DNA的相互作用  

Study on the Interaction between Rifampin and DNAby the Fluorescence Spectroscopy

作　　者：张爱梅[1] 王怀生[1] 孙盈[1] 苏兆安 张凌霞 

机构地区：[1]聊城大学 化学化工学院,山东聊城252059 [2]濮阳市第一高级中学,河南濮阳457000 [3]濮阳高新区实验学校,河南濮阳457000

出　　处：《聊城大学学报（自然科学版）》2007年第2期45-47,共3页Journal of Liaocheng University:Natural Science Edition

基　　金：国家自然科学基金(No.20475024);聊城大学科研基金(X061003)资助课题

摘　　要：在pH 7.4的Tris-HCl介质中,以盐酸小檗碱为荧光探针,研究了利福平与鲑鱼精DNA之间的相互作用.采用荧光光谱、吸收光谱、热变性等手段对作用机理进行了研究,结果表明主要作用方式为嵌入方式.求得结合常数为1.08×10~4L·mol^(-1),结合位点数为4.53.The interactions of rifampin （RFP） and sm-DNA have been studied by fluorescence spectroscopy using berberine （BR） as a fluorescence probe in Tris-HCl buffer slotion （pH 7.4）. The mechanism of the interactions between RFP and sm-DNA was also investigated by fluorescence spectrum,absorption spectrum, heat denaturation etc. The experimental results showed that the main interaction mode is the intercalation existed in this system. The average binding constants were determined as1.08× 10^4L mol^-1,and the most numbers of binding site were about 4.53,respectively.

关 键 词：荧光光谱法 DNA 利福平 小檗碱 

分 类 号：O657.32[理学—分析化学]