利迪链霉菌A02的抑菌谱及其抑菌活性的稳定性  

Determination on the antimicrobial spectrum and the stability of the antagonistic activity of Streptomyces lydicus A02

作　　者：隋勤[1] 刘伟成[2] 裘季燕[2] 刘霆[2] 潘争艳[1] 刘学敏[1] 

机构地区：[1]东北农业大学植保系,哈尔滨150030 [2]北京市农林科学院植物保护环境保护研究所,北京100097

出　　处：《植物保护》2007年第5期67-71,共5页Plant Protection

基　　金：国家自然科学基金项目(30671346);北京市自然科学基金项目(6072010);北京市科技计划项目(D0706005040231;D0705002040291)

摘　　要：离体抑菌试验表明,利迪链霉菌A02对植物病原真菌具有广谱的抑制活性,不同病原菌种类对其敏感性有一定的差异,果蔬灰霉病菌、番茄早疫病菌、小麦赤霉病菌和小麦纹枯病菌的敏感性较高;无菌发酵滤液稳定性测定显示,A02抑菌活性物质具有很好的热稳定性和耐酸碱、抗日光照射的特性,在70℃以下、pH5～8的条件下能较好地保持其抑菌活性。研究结果表明,拮抗菌A02在植物真菌性病害,尤其是半知菌引起的病害的生物防治中具有较高的应用价值。The antimicrobial activity and stability of Streptomyces lydicus A02 were determined. In the in vitro assay, strain A02 represented a broad antifungal spectrum against plant pathogenic fungi tested in the experiment. The four pathogens, Botrytis cinerea , Alternaria solani , Fusarium graminearium and Rhizoctonia cerealis , were especially sensitive to the strain. According to the tests on the filtrates from the fermented broth of strain A02, the anti- fungal substance produced by A02 displayed a higher stability to heat, light, acid and base. It could keep a good anti fungal activity under conditions of 70 ℃ and pH5 8. The results showed that S. lydicus A02 has a huge potential for further development in biocontrol of plant fungal diseases, especially those caused by fungi imperfecti.

关 键 词：利迪链霉菌 抑菌谱 生物活性稳定性 病原真菌 

分 类 号：S482.292[农业科学—农药学]