狐阴道加德纳氏菌病灭活疫苗免疫监测及效果评价  

Effect Evaluation and Immune Monitoring of Gardnerella Vaginalis of Fox Inactivated Vaccine

作　　者：闫喜军[1] 柴秀丽[1] 赵传芳[1] 邵西群[1] 王凤雪[1] 姜莉莉[1] 

机构地区：[1]中国农业科学院特产研究所,吉林吉林132109

出　　处：《特产研究》2007年第3期5-7,共3页Special Wild Economic Animal and Plant Research

基　　金：科技部科研院所社会公益研究专项(2004DIB4J159)

摘　　要：应用RBPT和Dot-ELISA方法对狐阴道加德纳氏菌病灭活疫苗免疫狐狸进行抗体监测。结果表明,疫苗接种第7d血清抗体阳转,第21～30d抗体达到高峰,高水平抗体可持续180d。通过免疫抗体与攻毒保护相关性测试,RBPT检测抗体在1:16和Dot-ELISA检测抗体在1∶320可抵抗GVF强毒菌株攻击获得保护,两种方法可以作为狐阴道加德纳氏菌病灭活疫苗效力检验评价方法在生产中应用。RBPT and Dot-ELISA was used to monitor antibody of foxes inoculated GVF inactivated vaccine, the results showed protective antibody appeared after 7 days of inoculation and antibody titer reached height after 21 - 30days, the high antibody titer mainrained 180 days. By testing interrelation of antibody and challenging protection, the foxes could resist GVF challenge when RBPT antibody was above 1：16 and Dot-ELISA antibody was above 1：320. The two methods might be applied to evaluate immune effect of Gardnerella Vaginalis of Fox Inactivated Vaccine

关 键 词：狐阴道加德纳氏菌病 灭活疫苗 免疫监测 效果评价 

分 类 号：S865.23[农业科学—野生动物驯养]