水通道蛋白3基因敲除小鼠的饲养繁殖及鉴定  

Breeding,reproducing and identifying for aquaporin-3 gene knock-out mice

作　　者：董春玲[1] 王桂芳[1] 肖奎[1] 李波[2] 马忠森[3] 白春学[1] 

机构地区：[1]上海复旦大学附属中山医院呼吸内科,上海200032 [2]吉林大学基础医学院解剖学教研室,吉林长春130021 [3]吉林大学第二医院呼吸内科,吉林长春130041

出　　处：《蚌埠医学院学报》2007年第5期505-507,共3页Journal of Bengbu Medical College

基　　金：国家自然科学基金资助项目(30370611)

摘　　要：目的:繁殖和鉴定水通道蛋白3(AQP-3)基因敲除小鼠。方法:将引进的杂合子小鼠进行饲养并繁殖,繁殖成功后其子代中将会出现野生型、杂合子以及纯合子3种基因型,提取每种基因型小鼠尾部基因组DNA,用PCR法进行鉴定,将其雄性纯合子与雌性杂合子交配,依照孟德尔遗传定律就有可能获得较多的基因敲除纯合子小鼠。结果:AQP-3杂合子小鼠的繁殖和饲养均获得成功,亦获得了较多的基因敲除纯合子小鼠。结论:正确的饲养繁殖以及鉴定方法是从杂合子中获得AQP-3基因敲除纯合子小鼠的有效途径。　Objective：To breed and identify aquaporin-3（AQP-3）gene knock-out mice.Methods：Heterozygote mice were bred and reproduced.Wild genotype,heterozygote genotype and homozygote genotype would appear in the offsprings of parents.Genome DNA extracted from the murine tails was subjected to PCR for genotype identification.Male homozygote mice were selected to mate with the female heterozygote mice to acquire homozygote baby mice according to Mendel law.Results：The breeding and reproducing were successful and more heterozygote genotype mice were reproduced.Conclusions：Appropriate methods of breeding,reproducing and identifying are effective ways for acquiring AQP-3 gene knock-out mice from heterozygote mice.

关 键 词：模型 生物学 水通道蛋白3 基因敲除 小鼠 

分 类 号：R318[医药卫生—生物医学工程]