两种荧光探针法研究三种非甾体抗炎药与DNA的相互作用  

Interaction of Three Kinds of Non-steroidal Anti-inflammatory Drugs with DNA Investigated by Two Fluorescence Probes

作　　者：杨昌英[1] 刘义[1] 曾芳[2] 李静[2] 李强国[1] 李林尉[1] 

机构地区：[1]武汉大学化学与分子科学学院,武汉430072 [2]三峡大学化学与生命科学学院,宜昌443002

出　　处：《化学学报》2007年第18期2076-2080,共5页Acta Chimica Sinica

基　　金：国家自然科学基金(Nos.30570015;20543004);湖北省创新群体(No.2005ABC02);教育部创新团队发展计划(No.[2006]8IRT0543);国家自然科学基金创新群体(No.20621502)资助项目.

摘　　要：采用溴化乙锭(EB)和中性红(NR)两种荧光探针分别考察了三种非甾体抗炎药(吲哚美辛、舒林酸和托美丁)与DNA的相互作用,采用荧光发射光谱和共振散射光谱技术得出了基本一致的结论:吲哚美辛和舒林酸可以在一定程度上与DNA结合(舒林酸更强),结合方式可能有嵌插作用,也可能有药物分子在DNA双链表面的组装,作用力来源于静电作用.而托美丁几乎不与DNA作用,因为其疏水性弱,与DNA分子结合几率小.The interaction of three chosen non-steroidal anti-inflammatory drugs （NSAID）, indomethacin, sulindac and tolmetin with calf thymus DNA has been investigated by fluorescence with the use of fluorescence probes, ethidium bromide （EB） and neutral red （NR）. When bound to DNA, sulindac and indomethacin could quench the fluorescence of EB at low concentration by exchanging with the EB probe, and attained saturation quickly. But sulindac and indomethacin continuously quenched the fluorescence emission and resonance light scattering spectra of NR in the presence of DNA until rather high concentration. These spectral characteristics supported the intercalation of NSAID into DNA as well as electrostatic assembly on the surface of DNA double strands.

关 键 词：非甾体抗炎药 DNA 荧光发射光谱 共振散射光谱 探针 

分 类 号：R96[医药卫生—药理学]