芽孢杆菌胞外产物对凡纳滨对虾蛋白酶活性影响的体外试验  

Effects of extracellular products of Bacillus licheniformis strain De on protease activity in white shrimp Litopenaeus vannamei in vitro

作　　者：曹煜成[1] 李卓佳[1] 冯娟[1] 

机构地区：[1]中国水产科学研究院南海水产研究所,广东广州510300

出　　处：《大连水产学院学报》2007年第5期372-376,共5页Journal of Dalian Fisheries University

基　　金：国家"十五"攻关项目(2004BA526B0202);国家"十一五"支撑计划项目(2006BAD09A07);广东重大科技专项项目(A3050304);广东省重大科技兴渔项目(B200201A01;A200601A01);珠海市科技计划项目(PC20061056)

摘　　要：用半透膜法收集地衣芽孢杆菌Bacillus licheniform isDe株的胞外产物(Extracellu lar products,ECP),以匀浆离心法提取凡纳滨对虾Litopenaeus vannam ei的肝胰腺和肠道消化酶,将ECP和对虾消化酶提取液按1∶9(体积比)混合,研究不同温度、pH条件下ECP对对虾蛋白酶活性的影响。结果表明:在不同温度、pH条件下,试验组与对照组没有显著性差异(P>0.05),即地衣芽孢杆菌ECP在体外条件下对对虾蛋白酶活性没有显著性影响;且试验组中芽孢杆菌ECP的添加量与其蛋白酶活性呈负相关。The extracellular products （ECP） collected by a semipermeable membrane from Bacillus licheniformis were mixed with digestive enzymes extracted by homogenization and centrifugation from white shrimp Litopenaeus vannamei hepatopanceas and intestine at a ratio of 1： 9. The effects of ECP on protease activity of the white shrimp were studied at various temperature and pH values in vitro. The results showed that there were no significant differences in protease activities between the test groups with the ECP and the control groups （ P 〉 0.05） at different temperature and pH. However, the inverse relationship was observed between the protease activities and the ECP quantity in the experimental groups.

关 键 词：地衣芽孢杆菌 胞外产物 凡纳滨对虾 蛋白酶 体外 

分 类 号：Q936[生物学—微生物学] Q55