cathpsin D在多囊卵巢综合征卵巢组织中的表达  

Cathepsin D expression in ovaries from polycystic ovarian sydrome patients

作　　者：金敏[1] 蔡婕[1] 胡燕军[1] 陆秀娥[1] 黄荷凤[1] 

机构地区：[1]浙江大学医学院附属妇产科医院,浙江杭州310006

出　　处：《浙江大学学报（医学版）》2007年第5期429-432,共4页Journal of Zhejiang University(Medical Sciences)

基　　金：2006年国家973项目(2006CB944006);浙江大学医学院科研启动基金资助项目

摘　　要：目的:检测cathepsin D蛋白在多囊卵巢综合征(polycystic ovarian syndrome,PCOS)患者卵巢组织中的表达。方法:采用Western blot免疫印迹法检测卵巢组织中cathepsin D蛋白的表达,并进行半定量分析;免疫组织化学法观察cathepsin D蛋白的分布定位。结果:PCOS组患者卵巢中cathepsin D蛋白表达显著低于对照组(2.06±0.39 vs 4.76±1.43,P<0.05);cathepsin D在卵泡和卵巢间质细胞上均有表达,其中在卵泡颗粒细胞上染色最深,主要定位在胞浆和胞膜。结论:cathepsin D蛋白在PCOS患者卵巢组织中表达水平显著下调,这可能是PCOS卵泡发育异常的机制之一。Objective： To investigate the expression of cathepsin D in ovary of patients with polycystic ovarian syndrome （PCOS）. Methods： Western blot was performed to detect the expression of cathepsin D and immunohitochemistry was used to detect the protein distribution in ovarian tissue. Results： Semi-quantity values of cathepsin D expression in PCOS and control group were 2.06±0.39 and 4.76±1.43（P〈0.05）,respectively. Immunostaining for cathepsin D was obvious in both follicles and stromal cells,and the strongest immunostaining was seen in granulosa cells of follicles. Immunochemical study showed the protein was mainly located on the cytoplasm and cell membrane. Conclusion： Cathepsin D expression is down-regulated in ovaries of PCOS patients,which may provide a clue for the abnormality of follicle development in PCOS.

关 键 词：多囊卵巢综合征/病理学 组织蛋白酶D/生物合成 免疫组织化学 受精 体外 胚胎移植 卵泡/生长和发育 粒层细胞 

分 类 号：R711.75[医药卫生—妇产科学]