分子修饰前后人参皂苷体外抗马立克氏病毒鸡胚成纤维细胞模型的建立  

Establishment of chicken embryo fibroblast model for ginsenoside with molecular modification against Marek's disease virus in vitro

作　　者：张煜[1] 褚秀玲[1] 苏建青[1] 郭志廷[2] 韦旭斌[2] 

机构地区：[1]河北北方学院动医系,河北张家口075131 [2]吉林大学畜牧兽医学院

出　　处：《中兽医医药杂志》2007年第5期19-22,共4页Journal of Traditional Chinese Veterinary Medicine

基　　金：国家自然科学基金(项目编号:30170698)

摘　　要：用雏鸡将马立克氏病毒(MDV)血毒复壮,分离发病鸡淋巴细胞并接种于鸡胚成纤维细胞,观察其病变。获得适应鸡胚成纤维细胞(CEF)的MD强毒,通过电镜观察、琼脂扩散实验进一步鉴定病毒,并建立了MDV感染CEF细胞模型,为研究人参皂苷及其衍生物的体外抗病毒作用及其机制奠定基础。The lymphocytes of the chickens,on which the Marek＇s disease virus（MDV） was rejuvenated,were isolated and inoculated into chicken embryo fibroblast（CEF）.The cell pathological effects were observed.MDV that could proliferate on CEF was obtained and confirmed through electron microscopic observations and agar diffusion assay.The chicken embryo fibroblast model was successfully established which would provide the bases for the further study on the antiviral effects and mechanism of ginsenoside and its derivatives in vitro.

关 键 词：人参皂苷 衍生物 马立克氏病毒 鸡胚成纤维细胞 

分 类 号：S855.3[农业科学—临床兽医学]