广西巴马小型猪动脉粥样硬化模型LOX-1表达的研究  

Study of lectin-like oxidized low-density lipoprotein receptor-1 expression in atherosclerotic of Guangxi bama minture swine

作　　者：余坚[1] 祖秀宏[2] 王宗保[1] 李宏光[3] 刘毅[3] 易光辉[2] 尹卫东[2] 

机构地区：[1]南华大学实验动物学部,湖南省衡阳市421001 [2]南华大学心血管病研究所,湖南省衡阳市421001 [3]韶关学院医学院生化教研室

出　　处：《中国心血管病研究》2007年第10期764-766,共3页Chinese Journal of Cardiovascular Research

基　　金：湖南省自然科学基金项目(05JJ20006;04JJ3058);湖南省科技厅科研项目(2006TP3015);湖南省卫生厅科研项目(ws-c2005034)

摘　　要：目的建立猪动脉粥样硬化(AS)模型,探讨该模型中LOX-1表达的变化。方法将10头广西巴马小型猪分别喂正常(CD)和高脂、高糖、高胆固醇(HFSCD)饲料,每月末测定血脂水平。第5个月末处死动物,采用苏丹Ⅳ检测动脉粥样斑块病变;逆转录-聚合酶链反应(RT-PCR)、Western Blot印迹分别检测主动脉组织中LOX-1的mRNA和蛋白质表达。结果与CD组比较,HFSCD组血浆甘油三酯、高密度脂蛋白胆固醇(HDL—C)和总胆固醇水平异常升高;动脉有明显的脂质条纹及斑块;动脉粥样硬化小型猪主动脉组织的LOX-1的mRNA及蛋白质的表达均上调(P<0.05)。结论高脂、高糖、高胆固醇饲料喂养小型猪可建立AS动物模型;AS小型猪主动脉组织的LOX-1表达上调。　Objective To study the expressions of lectin-like oxidized low-density lipoprotein receptor-1 in atherosclerotic mini swine and provide a new mechanism for investigating the pathogenesis of atherosclerosis. Methods Ten Guangxi Bama minipigs were randomized into two groups：control（CD）group（n=5）,fed with normal control diet）,high-fat/high-sucrose/high-cholesterol diet HFSCD group （n=5,fed with the high-fat/high-sucrose/high-cholesterol diet）. Plasma total cholesterol（TC）,high-density lipoprotein cholesterol（HDL-C）and triglyceride （TG）were examined at the end of every month. The expressions of LOX-1 mRNA and protein in aorta tissue were detected by reverse transcriptase-polymerase chain reaction（RT-PCR） and Western Blot. Results At the end of five months,plasma TC,HDL-C and TG in HFSCD mini swine were increased. There were obvious fatty streak and atherosclerotic plaque in mini swine aorta. The expression of LOX-1 was upregulated in HFSCD mini swine aorta tissue. Conclusion HFSCD may induce atheroselerosis and the expression ofLOX-1. Upregulating LOX-1 expression may promote atherosclerosis.

关 键 词：动脉硬化 脂蛋白类-LDL 猪 雏形 

分 类 号：R543.5[医药卫生—心血管疾病] Q95-33[医药卫生—内科学]