维生素E对CC1_4作用下HSC增殖和ECM影响  

Effect of vitamin E on proliferation of HSC injured by CCl_4

作　　者：高润平[1] 齐晓艳[1] 李广生[2] 

机构地区：[1]吉林大学第一医院感染症科,长春130021 [2]吉林大学地方病研究所

出　　处：《中国公共卫生》2007年第10期1175-1176,共2页Chinese Journal of Public Health

基　　金：吉林省自然科学基金(19990316;200505211)

摘　　要：目的观察维生素E(VE)对四氯化碳(CC14)作用下大鼠肝星状细胞(HSC)增殖和细胞外基质(ECM)产生的影响。方法采用原位灌注方法分离大鼠HSC。结果CC14作用下大鼠HSC3H-脯氨酸(3H-Pro)的掺入显著增加,Ⅲ型前胶原(PCⅢ)、透明质酸(HA)、板层素(LN)及纤维连接素(FN)的分泌水平明显提高。200nmol/LVE预处理4h可有效抑制CC14作用下大鼠HSC3H-胸腺密啶(3H-TdR)、3H-Pro的掺入,VE保护能使CC14作用下HSC分泌PCⅢ、HA、LN和FN减少与HSC产生丙二醛(MDA)下降,二者呈正相关。结论VE可抑制CC14作用下大鼠HSC增殖和通过抗氧化损伤抑制HSCECM的分泌。Objective To study the inhibitory roles of vitamin E（VE） on cell proliferation and extracellular matrix （ECM） synthesis of hepatic stellate cells （HSC） injured by Carbon Tetrachioride （CC14） in vitro. Methods Rat HSC were isolated and cultured. Results Addition of CC14 to HSC pretreated with 200 nmol/L VE 4 hours, the incorporation of ^3H - thymidine and ^3H - praline and the secretion of precollagen type Ⅲ, hyaluronic acid. laminin and fihronectin as well as production of malodialdehyde （MDA） were obviously inhibited. The analysis of correlation indicated that the effects of VE on inhibition of ECM secretion depended on lowering the production of MDA. Conclusion VE can inhibit the cell proliferation and secretion of ECM in rat＇s HSC injured by CC14 in vitro.

关 键 词：维生素E 肝星状细胞(HSC) 细胞外基质 

分 类 号：R977[医药卫生—药品]