多房棘球绦虫混合重组BCG-EmⅡ/3和BCG-Em14-3-3疫苗诱导小鼠脾细胞因子的变化  

Changes of cytokines of splenocytes in mice immunized by mix recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine of Em

作　　者：李文桂[1] 王鸿[1] 朱佑明[1] 

机构地区：[1]重庆医科大学附属第一医院传染病寄生虫病研究所,重庆400016

出　　处：《细胞与分子免疫学杂志》2007年第10期911-913,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目(30500423);教育部重点项目(205131);重庆市教委科研基金(KJ050313);重庆市科委科研基金(03-43-8);重庆市卫生局科研基金资助项目(03-2-099)

摘　　要：目的:探讨多房棘球绦虫混合重组BCG-EmⅡ/3和BCG-Em14-3-3疫苗免疫后再以Em原头节攻击后小鼠脾细胞因子的变化。方法:将疫苗采用皮下注射和鼻腔内接种分别免疫BALB/c小鼠后8周,用多房棘球绦虫原头节进行攻击感染。感染后18周杀鼠取脾,分离脾细胞,用EmAg或ConA刺激培养,并收集脾细胞培养上清液,用试剂盒检测脾细胞培养上清液中IL-2、IFN-γ、TNF-α和IL-4的水平,同时设有空载体、BCG和PBS对照。结果:疫苗接种组的IFN-γ和TNF-α水平升高,IL-4水平降低;皮下注射组的TNF-α水平高于鼻腔内接种组。结论:多房棘球绦虫混合重组BCG-EmⅡ/3和BCG-Em14-3-3疫苗可诱导小鼠产生Th1型细胞应答,抵抗Em原头节的攻击感染。疫苗皮下注射途径优于鼻腔内接种。AIM： To investigate the changes of cytokines of splenocytes in mice immunized by mix recombinant BCG-Em 11/3 and BCG-Eml4-3-3 vaccine of Echinococcus multilocularis（Em） and challenged by Em protoscoleces. METHODS： BALB/c mice were vaccinated subcutaneously and intranasally by the vaccine respectively. In the eighth week of vaccination they were Challenged by Em protoscoleces. In the eighteenth week of infection they were killed for spleen. After splenocytes were separated, their culture was stimulated by EmAg or ConA and their supematants were gathered. The levels of IL-2, IFN-γ, TNF-α and IL-4 were measured by ELISA kit. Blank vector, BCG and PBS were served as control. RESULTS： In the groups of vaccine immunization, the levels of IFN--γ and TNF-α increased obviously but the level of IL-4 decreased. The level of TNF-α in subcutaneous group was higher than that in intranasal group. CONCLUSION： Thl response was induced in mice immunized by mix recombinant BCG-Em 11/3 and BCG- Em14-3-3 vaccine of Em against the challenge by Em protoscoleces. Subcutaneous injection with this vaccine may be a good way of vaccination.

关 键 词：多房棘球绦虫 重组BCG—Em II/3疫苗 重组BCG.Eml4-3—3疫苗 细胞因子 

分 类 号：R383.3[医药卫生—医学寄生虫学]