乙型肝炎病毒携带者T淋巴细胞CD_(28)与免疫耐受的关系  

The Relationship Between CD_(28) of T-lymphocyte and Immune Tolerance in Hepastitis B Virus Carriers

作　　者：安萍，尹波，卞丽，吕丹，曹艳雪，吴星，张丽瑶 安萍[1] 尹波[1] 卞丽[1] 吕丹[1] 曹艳雪[1] 吴星[1] 张丽瑶[1] 

机构地区：[1]辽宁省沈阳市传染病院肝病研究所,110006

出　　处：《中国全科医学》2007年第20期1694-1696,共3页Chinese General Practice

摘　　要：目的探讨T淋巴细胞CD28表达与乙肝病毒携带(ASC)者免疫耐受的关系。方法采用流式细胞仪定量检测ASC者外周血及肝组织中CD28的水平;采用免疫组化技术检测ASC者肝组织CD28的表达率。结果不同Knodell积分的ASC者肝组织T淋巴细胞CD28表达情况间差别有显著性意义(P<0.05),ASC者中83.9%为无表达或低表达。ASC者外周血及肝组织中CD8+/CD2+8表达率分别为(20.0±5.8)%和(19.0±6.8)%,均较正常组低(P<0.05),且肝组织中CD8+/CD2+8水平较外周血下降更明显,差别有显著性意义(P<0.01)。肝组织中CD8+/CD2+8表达率随着肝组织学积分的减少而下降。结论ASC者肝组织CD28表达明显低下,T淋巴细胞不能完全活化,处于相对免疫耐受状态。ASC者肝组织中CD8+/CD2+8表达率较外周血更能反映肝脏免疫耐受状态。T淋巴细胞CD28表达与ASC者免疫耐受关系密切,且在其免疫耐受发病机制中起着重要作用。Objective To inquire into the relationship between CD28 of T - lymphocytes and immune tolerance in hepastitis B virus carriers. Methods Flow cytometry （FCM） was used to estimate quantitatively the changes of CD28 levels of peripheral blood and hepatic tissue in hepastitis B virus carriers; Expressions of CD28in hepatic tissue were determined by immuno - histochemical method. Results Significant difference was found in expression of CD28 of T - lymphocyte in hepatic tissus between different patients with different Knodell integrals of hepastitis B virus （ P 〈 0. 05 ） ; There was no expression or low expression in 83 9% of the patients ; The expression rates of CD8^＋/CD28^＋ in peripheral blood and hepatic tissues were respectively （ 20. 0 ± 5.8 ） % and （ 19. 0 ± 6. 8 ） %, lower than that of the normal group （ P 〈 0. 05 ） , and there was significant difference in levels of CD8^＋/CD28^＋ between hepatic tissues and peripheral （ P 〈0. 01 ） ; The expression of CD8^＋/CD28^＋ reduced with the decline of hepatic tissue integral. Condusion The expression of CD28 of hepatic tissues in patients with hepatitis B virus is significantly low, and T- lymphocyte can＇t be fully activated in a relative immune tolerance state. The expression of CD8^＋/CD28^＋ in hepatic tissues can reflect immune tolerance state more than that of peripheral blood. There is a close relation between expression of CD28 in T - lymphocyte and immune tolerance in patients with hepatitis B virus. Expression of CD28 in T - lymphocute plays an important role in immune tolerance pathologic mechanism.

关 键 词：免疫耐受 CD28 肝炎 乙型 乙型肝炎病毒携带 肝组织 外周血 

分 类 号：R512.62[医药卫生—内科学]