黑莓的组织培养快速繁殖技术  

Study on Tissue Culture and Rapid Propagation of Blackberry

作　　者：蒋小满[1] 柏新富[1] 赵建萍[1] 

机构地区：[1]鲁东大学生命科学学院,山东烟台264025

出　　处：《北方园艺》2007年第10期173-175,共3页Northern Horticulture

摘　　要：以黑莓茎尖和带芽茎段为外植体,筛选组织培养各阶段适宜培养基。结果表明:外植体以茎段为宜。初始培养基以MS+BA1.0mg/L+IAA0.1mg/L最好,出芽率达93%;继代增殖培养基以MS+BA1.0mg/L+NAA0.05～0.2mg/L最理想,增殖系数可达8倍;生根培养基以1/2MS+IBA0.1mg/L和1/2MS+NAA0.05mg/L最佳,生根率可达100%。以园土∶珍珠岩∶蚯蚓粪按1∶1∶1的比例混合的基质移栽成活率达到96.9%,移栽苗生长健壮。Using stem segments with buds as explants, tissue culture and rapid propagation of Blackberry were studied. The results indicated that the appropriate media for respective culture stages were： for germination of axillary buds： MS＋BA1.0mg/L＋IAA0. 1 mg/L; for multiplication： MS＋BA 1.0 mg/L＋NAA0. 05-0. 2mg/L, the multiple for multiplication reached to 8; for rooting： 1/2MS＋IBA0. 1mg/L or 1/2MS＋NAA0. 05mg/L and the rate of rooting was 96.3%. Transplanting rooted plantlet to a different substrata, the results showed that substrata which consists of garden -soil, perlite and wormcast was the most suitable for transplant and late growth, and survival rate was up to 96.9 %, and transplant plantlet grow healthy.

关 键 词：黑莓 组织培养 快速繁殖 植物激素 

分 类 号：S663.203.6[农业科学—果树学]