有机碘对人甲状腺细胞分泌细胞因子IL-6和IL-8的影响  

Effect of DIT on cytokines secretion in cultured human thyrocytes

作　　者：蔺新英[1] 赵妍[1] 姚苹[1] 郭冬梅[1] 

机构地区：[1]山东大学营养与食品卫生研究所,济南250012

出　　处：《中国地方病防治》2007年第5期343-345,共3页Chinese Journal of Control of Endemic Diseases

基　　金：山东省卫生厅科研基金资助项目(2001CA1AA18)

摘　　要：目的研究不同浓度的有机碘（DIT）对体外培养的人甲状腺细胞（TEC）分泌细胞因子IL-6和IL-8的影响，并且和无机碘（KI）进行比较，以探讨一种更安全有效的补碘制剂。方法取甲状腺腺瘤旁正常甲状腺组织，应用原代细胞培养技术进行细胞培养，以不同浓度（10^-8～10^-3 mol／L）的DIT和KI刺激单层培养的甲状腺细胞，采用双抗体夹心ELISA法测定各组甲状腺细胞培养上清液中细胞因子IL-6和IL-8的含量。结果正常的甲状腺细胞能分泌少量的IL-6和IL-8。DIT10^-6～10^-3 mol／L浓度组IL-6的分泌量高于正常对照组，10^-4 mol／L、10^-3 mol／L浓度组IL-6的分泌量低于同一浓度的KI组；DIT10^-5～10^-3 mol／L浓度组IL-8的分泌量高于正常对照组，10^-3 mol／L、10^-4 mol／L、10^-3 mol／L浓度组IL-8的分泌量低于同一浓度的KI组。在同一浓度上，DIT组IL-6和IL-8的增加程度低于KI组。结论高碘可刺激甲状腺细胞分泌更多的IL-6和IL-8，而有机碘的安全性相对高于无机碘。Objective To investigate the influence of DIT on cytokines （IL - 6, IL - 8） secretion of human thyrocytes and compare DIT with KI. To study a good kind of supplement to USI that is safe and effective, Methods The human thyroid epithelium cells from para- adenoma normal tissues from thyroid adenoma patients were cultured in the absence or presence of 10^-8 - 10^-3 mol/L DIT or KI. The contents of cytokines such as IL -6, IL -8 in supernatant of cultured thyrocytes were detected by ELISA method. Results A few IL - 6, IL - 8 were detected in supernatant of normal thyrocytes. IL - 6 in supernatant of DIT group （ 10^-6 - 10^-3 mol/L） increased significantly compared with normal thyrocytes. L - 6 in supernatant of DIT group （ 10^-4 mol/L, 10^-3 mol/L） decreased significantly compared with KI group （ 10^-4 mol/L, 10^-3 mol/L）. IL - 8 in supernatant of DIT group （ 10^-5 - 10^-3 mol/L） increased significantly compared with normal thyrocytes. IL - 8 in supernatant of DIT group （ 10^-5 mol/L, 10^-4 mol/L, 10^-3 mol/L） decreased significantly compared with KI group （ 10^-5 mol/L, 10^-4 mol/L, 10^-3 mol/L）. In the same contents, IL - 6, IL - 8 in supernatant of DIT group decreased significantly compared with KI group. Conclusion High concentration of iodine can increase cytokines （IL- 6, IL -8） secretion of normal thyrocytes. And DIT has more security than KI.

关 键 词：有机碘 人甲状腺细胞 细胞因子IL-6 细胞因子IL-8 

分 类 号：O613.44[理学—无机化学]