乙肝扶正胶囊的主要药效学研究  

Phamacodynamic Studies of Yiganfuzheng Capsule

作　　者：金翠英[1] 周建平[1] 马豹山[1] 李壮[2] 陈鸿珊[2] 

机构地区：[1]北京市药品检验所,北京100035 [2]中国协和医科大学医药生物技术研究所,北京100050

出　　处：《中国实验方剂学杂志》2007年第10期41-44,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:研究乙肝扶正胶囊的主要药效学作用。方法:选用D-半乳糖胺致小鼠急性肝损伤模型、卡介苗+脂多糖(BCG+LPS)造成小鼠免疫性肝损伤模型、四氯化碳(CCl4)致大鼠慢性肝损伤模型,研究乙肝扶正胶囊的保肝作用。选用鸭乙型肝炎病毒感染雏鸭,以分子斑点杂交法观察其对鸭血清乙型肝炎病毒DNA水平的影响。观察对环磷酰胺抑制免疫功能小鼠血清溶血素含量的影响。结果:乙肝扶正胶囊能明显降低D-半乳糖胺致急性肝损伤和BCG+LPS致免疫性肝损伤模型小鼠血清中ALT、AST的含量;对CCl4慢性肝损伤大鼠模型可降低血清中ALT、AST及肝羟脯氨酸含量,提高TP、ALB含量。对肝损伤均有明显的修复作用。对乙型肝炎病毒感染鸭血清DHBV-DNA水平抑制效果显著,拮抗环磷酰胺所致的体液免疫抑制,增加小鼠血清溶血素含量。结论:乙肝扶正胶囊有一定的保肝降酶、抑制鸭乙型肝炎病毒复制、提高机体免疫力的作用。Objective： To investigate pharmacological effects of Yiganfuzheng Capsule on liver. Method： The acute liver injury model, the immunological liver injury model, the chronic liver injury model were caused by D-galactosamine, BCG ＋ LPS and carbon tetrachloride, respectively. The ducklings were infected by hepatitis B virus. The dot-hybridization test was used to evaluate the duck hepatitis B virus＇ s DNA level in serum. The haemolysin test was used to investigate the effect on the immunosuppressive model caused by the cycophosphamide. Result：The Yiganfuzheng Capsule can markedly decrease the content of the ALT and AST in the serum of the acute liver injury model caused by D-galactosamine and by BCG ＋ LPS. Levels of ALT, AST in serum and hydroxyproline in liver were decreased, and the TP, ALB were increased, compared with the model group （ P 〈 0.01, P 〈 0.05） .It had inhibitory effect on the level of DHBV-DNA in the serum of duckling infected by hepatitis B. It could antagonize the humoral immunity inhibition caused by cyclophophamide, increased the level of serum haemolysin in mouse. Conclusion： Yiganfuzheng Capsule has effects of protecting liver, decreasing enzyme, inhibiting copy of duck hepatitis B virus, and enhancing the body immune function.

关 键 词：乙肝扶正胶囊 鸭乙型肝炎病毒 急慢性肝损伤 免疫性肝损伤 

分 类 号：R285.5[医药卫生—中药学]