HPLC法测定紫菀中槲皮素的含量  

Determination of Quercetin in Tatarian Aster(Aster tataricus)by HPLC

作　　者：夏运岳[1] 金卫坤[1] 陈幼亭[1] 吴爱勤[1] 

机构地区：[1]苏州医学院附属第一医院,215006

出　　处：《中草药》1997年第4期207-208,共2页Chinese Traditional and Herbal Drugs

摘　　要：用反相HPLC法测定了紫菀中槲皮素的含量。实验采用ODS柱，以0.4％磷酸溶液-甲醇（1：1）为流动相，360nm为检测波长，用外标法测定。回收率为97．39％，RSD0.39％，线性范围为8～40μg／mL。本法快速、简便、重现性好，可用于原材料的质量控制。Rp-HPLC method was developed for the determination of quercetin in Aster tataricus L. f.. ODS columnwas used with a mobile phase of methanol-0. 4% phosphoric acid(1: 1 )and detected at 360 nm. The flow ratewas 1.0 mL/min. External standard was used and the calibration curve was linear over the range 8～40 μg/mL(r=0. 9998). The extraction recovery was 97. 39% (RSD = 0. 39 % ). The method was simple, rapid, andwell reproducible. It can be used as a reliable method for the quality control of raw material.

关 键 词：紫菀 槲皮素 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]