多重脑震荡对大鼠空间认知行为的影响  

Effects of multiple cerebral concussion on rat spatial cognitive behavior

作　　者：江乐盛[1] 于建云[2] 高林波[1] 石泉[1] 李俊祥[1] 李娟娟[3] 

机构地区：[1]昆明医学院法医学院 [2]昆明医学院脑损伤研究室,云南昆明650031 [3]昆明医学院组胚教研室,云南昆明650031

出　　处：《中华神经外科疾病研究杂志》2007年第5期408-410,共3页Chinese Journal of Neurosurgical Disease Research

基　　金：国家自然科学基金资助项目(30560041);云南省自然科学基金资助项目(2005C0026Q);昆明医学院基金资助项目(kmc2005DG03)

摘　　要：目的探讨多重脑震荡(MCC)对大鼠认知行为的影响。方法成年SD大鼠24只,应用自制金属单摆式闭合性脑损伤机械打击装置复制MCC大鼠模型,随机分为对照组和MCC组,伤后应用Morris水迷宫实验,评价大鼠的空间学习记忆功能。结果在Morris水迷宫测试中,与对照组(8～14d测试结果分别为:64.74±23.43,33.16±17.70,36.59±27.53,37.60±18.20,37.14±26.03,16.85±10.63,10.02±6.51)相比,MCC大鼠伤后8～14d找到平台的时间明显延长,且在伤后的第9d(71.74±35.76)、第10d(63.14±31.02)、第13d(47.51±40.93)、第14d(42.39±43.33)差异具有显著性。在无平台探测实验中,与对照组(23.89±11.82)相比,MCC后第14d(15.12±6.56)大鼠在原有平台第三象限停留时间明显减少,差异显著(P<0.05)。结论实验性多重脑震荡大鼠在Morris水迷宫实验中,出现较为严重的空间认知行为障碍。Objective To study the effects of multiple cerebral concussion （MCC） on spatial cognitive behavior in rats. Methods Twenty-four Sprague-Dawley rats weighting between 280 g and 310 g were used. A MCC （one strike each day for continuous three days） rat model was produced using a simple pendulum device. One control and MCC groups were used at random （n =12 in each group）. The Morris water maze （MWM） was used to access spatial learning and memory function. Results Compared with the control group, during the 8 th and 14 th day after MCC, the latency of finding the platform was much longer and there were significant differences in the 9 th, 10 th, 13 th, 14 th day. Compared with the control group, probe trials demonstrated that the MCC produced reductions of staying time in the target quadrant, and there were statistically differences （P 〈 0.05） in the MCC group. Conclusion Experimental MCC present severe cognitive deficits in rats after multiple cerebral concussion.

关 键 词：多重脑震荡 认知行为 MORRIS水迷宫 大鼠 

分 类 号：R338.6[医药卫生—人体生理学]