大枣环磷酸腺苷(cAMP)提取工艺的研究  

Extract Process of Cyclic Adenosinemonophosphate(cAMP) in Ziziphus jujuba

作　　者：李明[1] 杨国林[1] 米沙[1] 高向耘[1] 王瑛[1] 李明润[1] 

机构地区：[1]天津市医药科学研究所,天津300020

出　　处：《中药材》2007年第9期1143-1145,共3页Journal of Chinese Medicinal Materials

基　　金：天津市科技攻关培育项目(NO.043121611)

摘　　要：目的:应用天津市静海县盛产的大枣生产cAMP,确定生产工艺,实现产业化。方法:应用水提、离子交换分离和硅胶G柱层析纯化。结果:提取物与对照品cAMP薄层色谱(TLC)的R f值一致(R f=0.75);提取物的紫外光谱(UV)与对照品cAMP在260 nm波长左右都有一个最大吸收峰,而在230 nm波长左右都有一个最小吸收值;提取物的红外光谱(IR)与对照品cAMP的标准红外光谱〔1〕一致;高效液相色谱(HPLC)测定提取物的保留时间为5.237分钟,未出现杂质峰,含量为97%,对照品cAMP的保留时间为5.350分钟,二者保留时间存在微小差异。结论:这一物质的化学结构与对照品cAMP相同。Objective ： To study the extract process of Cyclic adenosinemonophosphate （ cAMP ） in Ziziphus jujuba. Methods ： CAMP was extracted with water,separated and purificated by ion exchange and silica gel G column chromatography. Results ： Thin layer chromatography （TLC） of the extracts and control cAMP showed the same Rf 0. 75 ; The UV-absorption of the extracts and control cAMP had maximal absorb peak at 260 nm and the least absorb value at 230 nm;Infra-red spectrum of the extracts was indistinguishable from that of control cAMP; Retention time of the extracts by high-performance liquid chromatography （HPLC） determinate was 5. 237 minute, unsettled impurity peak ,content was 97%. Control cAMP was 5. 350 minute, their retention time were of little difference. Conclusion： Chemistry structure of this extract with that of control cAMP is the same.

关 键 词：大枣 离子交换分离 CAMP 

分 类 号：R284.2[医药卫生—中药学]