花生空间诱变及SSR标记遗传多态性分析  

Study on breeding of space mutants in peanut and analysis of genetic diversity based on SSR marker

作　　者：周桂元[1] 洪彦彬[1] 林坤耀[1] 李少雄[1] 梁炫强[1] 

机构地区：[1]广东省农业科学院作物研究所,广东广州510640

出　　处：《中国油料作物学报》2007年第3期238-241,共4页Chinese Journal of Oil Crop Sciences

基　　金：广东省科技计划项目(2003B21203;2006A20201003);国家863项目(2001AA241153)

摘　　要：以返回式卫星搭载花生品种粤油7号种子为材料,经过3年地面种植和选择,获得21个变异株系。选用110对SSR引物对21个变异株系进行SSR多态性分析,有5对引物的扩增在变异株系与对照品种之间表现出多态性。说明太空能够诱导花生种子发生基因变异,空间诱变可作为花生种质资源创新和品种选育的方法之一。The seeds of a peanut variety, Yueyou No. 7, were carried into outer space by a recoverable satellite in 2003. The seeds were grown and screened for mutants. 21 mutant lines were obtained. SSR （simple sequences repeat ） analysis was performed between these mutant lines and its original variety. Total 110 pairs of primers were screened, 5 of them amplified polymorphic products. The contrast between 21 mutant lines and the original variety showed polymorphism in their SSR pattern. These results indicated that the outer space mutation would be an effective method to develop new peanut germplasm.

关 键 词：花生 航天育种 SSR 

分 类 号：S565.202.4[农业科学—作物学]