丙型肝炎抗体筛检异同性与 EIA 试剂质量评价方法探析  

Evaluation on the Similarities and Differences of Anti HCV Screening and the EIA Reagents Qualities

作　　者：赵炳华[1] 

机构地区：[1]浙江医科大学附属第一医院

出　　处：《预防医学情报杂志》1997年第2期75-78,共4页Journal of Preventive Medicine Information

摘　　要：本文报道采用和参照检测２ＮＣＵ／ｍｌ抗ＨＣＶ定值血清Ｃ．Ｏ．Ｉ．是否＞１．０、以及检测ＨＣＶ血清盘按预期结果计算和评价真实性和预示值及调整一致性和约登指数等各项指标，比较与血清盘预期结果列为假阴性和假阳性结果的不一致性和错位现象，计算与比较检出血清盘阳性和阴性ＯＤ值的Ｘ—±２ＳＤ与Ｃ．Ｏ．值的差值和比值，表明商售抗ＨＣＶＥＩＡ药盒检测灵敏度和特异度有不同的两种类型，提示供血筛检和临床免疫诊断，宜各取高灵敏度和高特异度两种厂牌试剂，采用配对并联筛检试验方案作双次检测，以减少漏检和误诊。To investigate the similarities and differences on the HCV antibodies screening, 2 NCU/ml anti HCV critical value serum and HCV sera panel were detected by five commercial EIA kits, and the C.O.I.(cut off index, i.e. sample OD/cut off OD ratio.), sensitivity and specificity, crude and adjusted agreement, and Youden's index, predictive values of a positive and a negative test, and the distance and ratio of the positive and negative sera OD X— /+ 2SD from the cut off value among the HCV sera panel were calculated. These results suggested that the sensitivity and specificity had its strong and weak points among the EIA Kits detected, and recommend that the screening in parallel with the different manufacturer's Kits was used for serosurveillance and/or sero immunological diagnosis in order to reduce the proportion of false negative or positive.

关 键 词：丙型肝炎 抗-HCV EIA试剂 

分 类 号：R512.630.4[医药卫生—内科学] R446.62[医药卫生—临床医学]