茶藨生柱锈重寄生木霉分生孢子萌发的生物学特性  

Biological Characteristics of Conidiospores Germination Three Trichoderma Isolates

作　　者：陈玉惠[1] 周利[1] 李永和[1] 林宏益[1] 

机构地区：[1]西南林学院保护生物学院,云南昆明650224

出　　处：《南京林业大学学报（自然科学版）》2007年第5期53-56,共4页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：云南省自然科学基金资助项目(2002C0047M);云南省科技攻关资助项目(2003NG12)

摘　　要：3株茶藨生柱锈菌（Cronartium ribicola J.C.Fischer）重寄生木霉（Trichoderma spp.）（TR1、TR2、TR3）的分生孢子在水琼脂、葡萄糖、PDA、改良Fries、华山松树皮浸汁和锈孢子培养基上均能萌发,TR1在各种培养基上的萌发率最高,萌发速度最快,而TR2和TR3在水琼脂和葡萄糖培养基上的萌发则较差;3个菌株孢子萌发的最适温度范围为20-30℃,其中TR1的萌发温度范围最广,在15-35℃内均能较好地萌发;3种孢子萌发的最适pH范围为4-5,相对湿度范围35%-100%（50%以上较易萌发）;黑暗和交替黑暗能刺激TR1孢子萌发,连续光照则有利于TR2和TR3孢子的萌发。Conidiospores of Cronartium ribicola mycoparasites Trichoderrna spp. including TR1,TR2, TR3 could germinate in each culture medium of agar plate, glucose, PDA, Fries, bark juice of Pinus armandii and aeciospores. Strain TR1 showed its germination activity and rate on all mediums was the highest, whereas strain TR2 and strain TR3 germinated less on the medium of agar plate and glucose. The optimum temperature for conidiospore germination of three mycoparasites ranged from 20℃ to 30℃ and TR1 had the most wide temperature ranging from 15℃ to 35℃. The optimum pH for conidiospore germination of three isolates was 4.0-5.0 with the relative humidity from 35% to 100%. Conidiospores could germinate easily with the relative humidity above 50%. Dark and interval dark could stimulate conidiospore germination of TR1. However, continuous exposure to light contrib uted to spore germination of TR2 and TR3.

关 键 词：木霉菌株 孢子萌发 生物学特性 茶藨生柱锈菌 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]