环氧乙烷对大鼠DNA甲基化酶活性的影响  

Effects of Ethylene Oxide on Activity of DNA Methylase in Rats

作　　者：钟先玖[1,2,3] 张龄[1,2,3] 张民 周元陵[1,2,3] 范卫[1,2,3] 余龙 

机构地区：[1]上海医科大学金山医院职业病防治科 [2]上海职工医学院 [3]复旦大学遗传研究所

出　　处：《中华预防医学杂志》1997年第3期160-162,共3页Chinese Journal of Preventive Medicine

基　　金：国家自然科学基金

摘　　要：为探讨环氧乙烷（Ｅｔｏ）对细胞内主要修饰酶ＤＮＡ甲基化酶活性的影响，对Ｗｉｓｔａｒ雄性大鼠采用静式吸入染毒，浓度７５１．７ｍｇ／ｍ３，每天２小时，每周６天，连续染毒９周。对其肝、肾和睾丸ＤＮＡ进行限制性内切酶同裂酶（ＭｂｏＩ和Ｓａｕ３ＡＩ，ＨｐａⅡ和ＭｓｐⅠ）敏感性试验和Ｓｏｕｔｈｅｒｎ印迹杂交分析。结果：大鼠肝、肾和睾丸ＤＮＡ经ＨｉｎｄⅢ、ＭｂｏⅠ和Ｓａｕ３ＡⅠ酶解后呈完全酶解带型，仅见１条杂交带。经ＨｐａⅡ和ＭｓｐⅠ酶解后呈不完全酶解带型，均见多条杂交带。电泳带型、杂交带条数及放射自显影强度在染毒组和对照组之间差异均无显著性。表明Ｅｔｏ对大鼠肝、肾、睾丸ＤＮＡ甲基化酶活性无影响，睾丸ＤＮＡ甲基化程度远低于肝和肾。Male Wistar rats were exposed to ethylene Oxide (Eto) via inhalation at a concentration of 751.7 mg/m 3 two hours a day and six days a week for nine consecutive weeks. Sensitivity test of restriction enzymes MboⅠ, Sau3AⅠ, HpaⅡ and MspⅠ and Southern blot were conducted to study the effects of ethylene oxide on activity of major intracellular modification enzyme, DNA methylase. Results showed that DNA in liver, kidney and testis of rats were completely enzymolysed and only one blot was seen after being treated with HindⅢ, MboⅠ and Sau3AⅠ, and incompletely enzymolysed and multiple blots were seen after being treated with HpaⅡ and MspⅠ. There were no significant differences in electrophoresis pattern, number of blots and intensity of radioautograph between the exposed animals and controls. It indicated that no effects of Eto on activity of DNA methylase in the liver, kidney and testis of rats was found, and degree of methylation in the testis was much less than that in the liver and kidney.

关 键 词：环氧乙烷 大鼠 DNA 甲基化酶 活性 

分 类 号：R992[医药卫生—毒理学]