苏太猪Mx1基因第14外显子多态性及其与繁殖性能的关联分析  

Polymorphism of the Exon 14 of Mx1 Gene and Its Relationship with Reproductive Performance in Sutai Pigs

作　　者：吴圣龙[1] 包文斌[1] 鞠慧萍[1] 黄雪根 华金第 陈国宏[1] 

机构地区：[1]扬州大学动物科学与技术学院,扬州225009 [2]江苏省苏州市苏太猪育种中心,苏州215128

出　　处：《畜牧兽医学报》2007年第10期1015-1020,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：江苏省高技术研究(农业)(BG2006302)

摘　　要：采用PCR-RFLP方法对苏太猪Mx1基因第14外显子的多态性进行分析,PCR产物经Hin6Ⅰ酶切后,共检测到3个等位基因,6种基因型。卡方检验结果表明,苏太猪在Mx1基因Hin6 I酶切位点已经达到Hardy-Weinberg平衡状态。基因型为BC的种母猪第3胎总产仔数、产活仔数、初生窝重、断奶仔猪数和断奶窝重均显著高于基因型为BB的个体(P<0.05),也高于基因型为AA、AB、AC和CC的个体,但是差异不显著(P>0.05)。通过测序在扩增片段中新发现了3种类型的碱基突变,前2个分别导致了Thr和Glu向Ala和Arg的替换,最后一个突变不引起氨基酸的变化,且后两个突变位点为BB基因型所特有;本研究提示Mx蛋白Glu→Arg的突变位点可能与其抗病毒能力有关。Polymorphisms in exon 14 of Mx1 gene were detected by PCR-RFLP in Sutai pigs. Six genotypes and three alleles were observed when exon 14 of Mx1 gene was digested by Hin6 Ⅰ restriction enzyme. The results of Chi Square test indicated that the frequencies patterns of exon 14 of Mx1 gene were fit with Hardy-Weinberg equilibrium in Sutai pigs population. Correlation between genotypes and reproductive performances （total litter size, number born alive, birth litter weight, number weaning piglets and weaning litter weight in the third litter） of Sutai pigs was similarly analyzed. The results indicated that reproductive performances of Sutai pigs with BC genotype were significantly （P〈0.05）higher than those with BB, AA, AB, AC and CC genotypes. Three new points mutation were identified by sequencing PCR products, two of which r suited in Thr→Ala and Glu→Arg were missense mutation, and the last was a silence mutation. Glu→Arg point mutation and silence mutation were appeared in the individuals with BB genotype. The results also indicated that the point mutation Glu→Arg may influence the virus resistance of Mx protein in pigs.

关 键 词：猪 Mx1基因 繁殖性能 

分 类 号：S828.2[农业科学—畜牧学]