用溶血素从感染红细胞分离羊泰勒虫裂殖子  

Purification of Merozoites of Theileria spp. from Infected Erythrocytes in Sheep Using Hemolysin

作　　者：李有全[1] 罗建勋[1] 关贵全[1] 马米玲[1] 高金亮[1] 刘志杰[1] 党志胜[1] 刘爱红[1] 刘军龙[1] 任巧云[1] 鲁炳义[1] 白启[1] 殷宏[1] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室/甘肃省动物寄生虫病重点实验室,兰州730046

出　　处：《畜牧兽医学报》2007年第10期1093-1097,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家"863"计划(2006AA10A207);国家自然科学面上项目(30571397);欧盟EPIZONE;ICTTD和SSA-INCOME项目(INCONo.510561)

摘　　要：用嗜水气单胞菌产生的细胞毒素—溶血素Ah-1,裂解羊泰勒虫感染的红细胞,通过Percoll密度梯度离心分离羊泰勒虫裂殖子,建立了一种自感染红细胞中分离泰勒虫裂殖子的快速方法。光学显微镜观察显示,所分离的羊泰勒虫裂殖子基本保留原有的正常形态,并从宿主细胞成分中游离。A simple and rapid method for purifying merozoites of Theileria spp. from infected erythrocytes in sheep or goats was developed. Infected erythrocytes were effectively lysed using hemolysin Ah-1 produced by Aeromonas hydrophila, and the lysate was subjected to centrifugation in a Percoll discontinuous density gradient at common speed. Light microscopical study showed that the merozoites purified retained their intact basic structure and were free of infected erythrocyte membrane and other host-cell components.

关 键 词：羊泰勒虫 裂殖子 溶血素 嗜水气单胞菌 

分 类 号：S852.72[农业科学—基础兽医学]