肉犊牛小肠黏膜免疫相关细胞的数量变化研究  

Study of the Population of Cells Associated with Small Intestinal Mucosal Immunity in Beef Calves

作　　者：宋恩亮[1] 陈耀星[2] 王子旭[2] 王文利[3] 刘晓牧[1] 徐西彬[2] 巢国正[2] 吴乃科[1] 

机构地区：[1]山东省农业科学院畜牧兽医研究所山东省畜禽疫病防治与繁育重点实验室,济南250100 [2]中国农业大学动物医学院,北京100094 [3]北京农业职业学院,北京102442

出　　处：《畜牧兽医学报》2007年第10期1109-1114,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目(30471247);山东省农科院畜牧所青年基金(2004);山东省农科院青年基金(2006YQN031)

摘　　要：分别选择1、4、6月龄各4头利杂犊牛的十二指肠、空肠、回肠,利用组织化学法和图像分析法研究犊牛小肠的上皮内淋巴细胞、杯状细胞和肥大细胞的数量变化。结果显示:在同一月龄犊牛的小肠的上皮内淋巴细胞数量由十二指肠向空肠、回肠逐渐减少,而杯状细胞的数量逐渐增加,肥大细胞的密度逐渐降低。在1、6月龄犊牛十二指肠、空肠、回肠的上皮内淋巴细胞数量之间差异极显著(P<0.01);4月龄十二指肠的上皮内淋巴细胞数量最多(P<0.01)。在1月龄犊牛十二指肠、空肠、回肠杯状细胞的数量差异极显著(P<0.01);4和6月龄犊牛十二指肠和空肠之间的杯状细胞的数量差异不显著(P>0.05),但二者与犊牛回肠杯状细胞的数量差异极显著(P<0.01)。各年龄犊牛十二指肠、空肠、回肠的肥大细胞密度差异极显著(P<0.01),4月龄时犊牛小肠的肥大细胞密度最低。结果提示犊牛的黏膜免疫水平可能与其机体的发育相一致。The objective of this study was to investigate the changes of the number of lymphocyte, goblet cell reside within the intestinal musocal epithelium and mast cells＇ density within intestinal wall area in beef calf. Histochemical and the Scion image software analysis techniques were used. Data were collected from duodenum, jejunum and ileum of 4 beef Limousin crossbred calves at 1-, 4- and 6-month of age, respectively. Results showed that intraepithelium lymphocyte number and mast cells＇ density gradually decreased, but goblet cell number gradually increased from duodenum, jejunum to ileum in beef calf at same month of age. Intraepithelium lymphocyte number of duodenum, jejunum and ileum in beef calf, which significantly differed （P〈0. 01）each other at 1- and 6-month of age, intraepithelium lymphocyte number of duodenum at 4-month of age was the most. Goblet cell number of duodenum, jejunum and ileum in beef calf was significantly different（P〈0. 01）each other at 1-month of age. At 4- and 6-month of age, goblet cell number didn＇t differ significantly（P〉0.05） between duodenum and jejunum, but the numbers of these two seg- ments were less （P〈0.01）than that of ileum. As for mast cells＇ density within intestinal wall area of duodenum, jejunum and ileum in beef calf, they significantly differed （P〈0.01）each other at 1-, 4- and 6-month of age, respectively, mast cells＇ density within intestinal wall area was least at 4-month of age. Our results indicated immune responses may involve the coordination and potentially redundant development of calf.

关 键 词：肉犊牛 黏膜免疫 上皮内淋巴细胞 杯状细胞 肥大细胞 

分 类 号：S852.16[农业科学—基础兽医学]