中国美利奴羊MHC-DRB_1基因PCR-RFLP多态性分析  

Polymorphism Analysis of MHC-DRB_1 Gene in Chinese Merino Sheep by PCR-RFLP

作　　者：彭林泽[1] 申红[1] 贾斌[1] 余智勇[1] 曾现存[1] 刘宏坤 彭波 陈明辉 郭玉强 

机构地区：[1]石河子大学动物科技学院,石河子832003 [2]新疆伊犁农四师74团畜牧兽医站,伊犁835600 [3]新疆塔城农九师畜牧兽医站,塔城834600

出　　处：《畜牧兽医学报》2007年第10期1115-1119,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金资助项目(30660124);国家教育部"高等学校博士学科点专项科研基金资助课题"(20050759001)

摘　　要：应用巢式PCR-RFLP方法,对211只中国美利奴(新疆军垦型)羊的MHC-DRB1外显子2的遗传多态性进行检测,并对基因型频率和等位基因频率进行了统计,结果表明,中国美利奴(新疆军垦型)羊的MHC-DRB1基因外显子2在SacⅠ、Hin1Ⅰ和HaeⅢ的酶切位点存在多态性,这些酶切位点分别受2、2和6个等位基因控制,由于MHC-DRB1为多碱基突变的基因位点,综合3种限制性内切酶的酶切结果,在中国美利奴(新疆军垦型)羊中共发现24种等位基因;χ2适合性检验结果表明,中国美利奴(新疆军垦型)羊的MHC-DRB1外显子2在SacⅠ和Hin1Ⅰ酶切位点均达到Hardy-Weinberg平衡(P>0.05),但在HaeⅢ酶切位点未达到Hardy-Weinberg平衡(P<0.01)。The polymorphism of the exon 2 of MHC-DRB1 was investigated by nested PCR-RFLP technique at three loci digested with Sac Ⅰ ,Hinl Ⅰand Hae Ⅲ ,respectively, in a total number of 211 individuals of Chinese Merino（Xinjiang Army Wasteland Reclamation） sheep, and genotypic frequencies and allelic frequencies were counted. The results showed that the extensive polymorphisms of the exon 2 of MHC-DRB1 were found at three loci digested with Sac Ⅰ , Hinl Ⅰ and Hae Ⅲ ,respectively, in Chinese Merino sheep, these sites were controlled by two, two and six alleles, respectively. As some base mutations were found in MHC-DRB1 locus, and according to the results in this paper, twenty-four alleles were found in Chinese Merino （Xinjiang Army Wasteland Reclamation） sheep. The results of Xz test showed that genotypes of MHC-DRB1 digested with Sac Ⅰ and Hinl Ⅰ except HaeⅢ （P〈0.01）fitted with Hardy-Weinberg equilibrium（P 〉0. 05）.

关 键 词：中国美利奴羊 MHC-DRB  PCR—RFLP 

分 类 号：S826.862[农业科学—畜牧学]