细胞表面β1,4-半乳糖基转移酶1功能研究进展  

RECENT ADVANCES IN β1,4-GALACTOSYLTRANSFERASE 1

作　　者：丁怡甜[1] 辛现良[1] 耿美玉[1] 

机构地区：[1]中国海洋大学海洋医药学院,山东青岛266003

出　　处：《生物物理学报》2007年第5期351-356,共6页Acta Biophysica Sinica

基　　金：国家重点基础研究发展规划(973)资助项目(2003CB716400)~~

摘　　要：β1,4-半乳糖基转移酶1(β1,4-galactosyltransferase1,β4GalT1)是人们研究最广泛、最深入的糖基转移酶之一。β4GalT1的基因编码了长形和短形两种蛋白,长形的=βGalT1比短形的β4GalT1在胞浆区多了13个氨基酸的尾巴,正是由于这种差异造成了β4GalT1定位以及功能上的不同,短形的βGalT1和部分长形的β4GalT1分布在高尔基体上,发挥糖基转移酶的基本功能,还有一部分长形的β4GalT1分布在细胞表面,发挥粘附分子样作用,在精卵结合、神经突触生长、神经元迁移、肿瘤细胞迁移等过程中有非常重要的意义。文章主要从β4GalT1的基本结构、β4GalT1与多种配体之间的相互作用介导了细胞不同的生物学功能、β4GalT1与细胞骨架蛋白的关系等几个方面综述细胞膜表面F4GalT1的研究进展。β1,4-galactosyltransferase （β4GAIT1） is a Goigi-resident type II membrane enzyme. The gene for β4GalT1 encodes two polypeptides, long and short, which differ by a 13 amino acid extension on the amino terminal end of the long isoform. With the use of human multiple tissue Northern blots β4GalT1 transcript has been shown to be present in most tissues except for brain, lung and small intestine, which contained little transcript and its expression is enhanced in the mammary gland during lactation. It is an amazing enzyme, mediating cellular interaction during sperm： egg binding, embryonic cell-cell adhesion, cell migration, neurite outgrowth and et al. It resides in two distinct subcellular locations, where it performs different functions. All short and most long β4GalT1 are localized in the trans-Goigi complex, where it participates in cellular glycosylation. A subpopulation of β4GalT1 is also present on the plasma membrane, where it binds to specific glycoside residues on multiple extracellular ligands, and mediates cell-cell as well as cell-matrix interactions for a variety of cells. When expressed on the surface, β4GalT1 associates with the actin cytoskeleton and, upon ligand-induced aggregation, induces cell-type specific intracellular signal cascades. The following review on β4GalT1 focused on the interaction with multiple extracellular ligands and the cytoskeleton.

关 键 词：β1 4-半乳糖基转移酶1 配体 细胞骨架 

分 类 号：R555.4[医药卫生—血液循环系统疾病]