Investigation of Function of Novel Sperm Binding Protein HBRP in Human  

作　　者：Jie ZHANG Ying LIU Wen-hui SU Guo-li WANG Zhi-hong ZONG Bing-zhi YU 

机构地区：[1]Department of Biochemical and Molecular Biology, China Medical University, Shenyang 110001, China

出　　处：《Journal of Reproduction and Contraception》2007年第3期173-180,共8页生殖与避孕（英文版）

基　　金：This research was supported by funds from the National Natural Science Foundation (30170987) of China

摘　　要：Objective To investigate the biology function of novel protein related to bovine seminal plasma protein in human testis. Methods Recombination pcDNA3/HBRP was constructed and transfected to HEK293 cell and permanently expression cell line was established. The activity of protein kinase C （PKC） of the cell line was detected by autoradiography method. Results The stable expression cell line of HBRP was obtained. The HBRP inhibited the activity of PKC significantly. Conclusion One of the new functions of novel sperm binding protein in human is the inhibitor action on activity of PKC. It may be involved in the sperm capacitation and acrosome reaction.Objective To investigate the biology function of novel protein related to bovine seminal plasma protein in human testis. Methods Recombination pcDNA3/HBRP was constructed and transfected to HEK293 cell and permanently expression cell line was established. The activity of protein kinase C （PKC） of the cell line was detected by autoradiography method. Results The stable expression cell line of HBRP was obtained. The HBRP inhibited the activity of PKC significantly. Conclusion One of the new functions of novel sperm binding protein in human is the inhibitor action on activity of PKC. It may be involved in the sperm capacitation and acrosome reaction.

关 键 词：HBRP eukaryotic expression PKC activity 

分 类 号：R339.21[医药卫生—人体生理学]