RP-HPLC法测定小儿肺炎合剂中黄芩苷含量  

Determination of baicalin in the children's pneumonia mixture by RP-HPLC

作　　者：陈兆军[1] 李中[2] 王栋[2] 

机构地区：[1]辽宁省大连市儿童医院药剂科,大连116011 [2]辽宁省大连市皮肤病医院,大连116011

出　　处：《海峡药学》2007年第9期31-33,共3页Strait Pharmaceutical Journal

摘　　要：目的建立小儿肺炎舍剂中黄芩苷的RP—HPLC含量测定方法，为制定质量标准提供依据。方法采用Symmetry C18柱（3．9×150mm，5μm）；流动相为甲醇-水-磷酸-三乙胺（50：40：0．2：2）；柱温：38℃；流速1mL·min^-1；检测波长277nm。结果黄芩苷在进样量为0．020～1．000μg范围内线性关系良好（r=0．9999），平均加样回收率为99．89％，RSD=1．02％。结论所建立的方法灵敏度高，可靠性好，操作方便，可作为肺炎合剂的质量控制方法。OBJECTIVE To establish a determination method of baicalin in the children＇s pneumonia mixture by RP-HPLC,and to set up its quality control standard. METHODS A Symmetry C18 column was used with methanol-water-phosphoric acid-triethylamine （ 50： 40： 0. 2： 2 drops） as mobile phase, column temperature was at 38 ℃. The flow rate was 1mL·min^-1. The content was detected at the wavelength of 277nm. RESULTS The qualificationmethod of the baicalin had the linear range of 0. 020 ～ 1. 000μg（ r = 0. 9999）. The average recovery was 99.89% with RSD of 1.02%. CONCLUSION The method for quantification was sensitive reliable and convenient for quality control of children＇s pneumonia mixture.

关 键 词：RP—HPLC 小儿肺炎合剂 黄芩苷 含量测定 

分 类 号：R927.2[医药卫生—药学]