高效液相色谱法测定呋噻米注射液的含量  

Determination of Content of Furosemide Injection by HPLC

作　　者：庄阳辉[1] 石炳光[1] 

机构地区：[1]福州市第二医院,福州350007

出　　处：《海峡药学》2007年第9期33-34,共2页Strait Pharmaceutical Journal

摘　　要：目的建立高效液相色谱法测定定呋噻米注射液含量的方法。方法C18（150mm×4．6mm，5μm）色谱柱，以水一甲醇．磷酸盐缓冲液（50：49：1）为流动相，流速1．0ml·min^-1，检测波长为275nm。结果呋噻米在0．049～0．2921μg范围内进样量与峰面积呈良好的线性关系（r=0．9999）；平均回收率为98．94％，RSD为1．24％，n=9。结论本方法结果准确，重现性较好。OBJECTIVE To establish a method for determination content of furosemide injection by HPLC. METHODS C18 column was used and water-ethanol-phosphate buffer（50： 49：1 ）was used as mobil phase,the flow rate was 1.0ml · min^-1 ,the detecting wavelength was at 275nm. RESULTS There was a good linear relationship between the concentration of furosemide and peak area within a range of 0. 049 ～ 0. 292μg ,r = 0. 9999. The average recovery was 98.94% with RSD = 1.24% （ n = 9）. CONCLUSION The method is highly repeatable and accurate.

关 键 词：含量 呋噻米 注射液 HPLC 

分 类 号：R927.2[医药卫生—药学]