HPLC测定人血浆中醋氯芬酸药物浓度的研究  

Determination of Aceclofenac in Human Plasma by HPLC

作　　者：尹洪银[1] 高希宝[1] 禚金花[1] 付军[1] 

机构地区：[1]山东大学公共卫生学院,山东济南250012

出　　处：《食品与药品》2007年第10A期16-18,共3页Food and Drug

摘　　要：目的建立测定人血浆中醋氯芬酸浓度的高效液相色谱法。方法取血浆500μL酸化后用乙酸乙酯一步提取,40℃水浴氮气吹干,流动相复溶后进样测定。采用HypersilC18柱(250mm×4.6mm,5μm);流动相为甲醇-0.1%冰醋酸(65:35,V/V),流速1.2mL/min;检测波长为276nm,柱温为室温。结果醋氯芬酸的血药浓度在0.1～15.0μg/mL范围内线性关系良好,此方法的最低检测浓度为0.1μg/mL。血浆中低、中、高3种浓度的回收率分别为103.23%,104.33%,101.11%,精密度RSD日内为2.800%～6.841%,日间为4.702%～8.161%。结论该方法专属性强,重现性好,操作简便。适用于药动学研究和血药浓度监测。Objective To establish a HPLC method for the determination of aceclofenac in human plasma. Methods Acidified samples of plasma （500 μL） were extracted with 4 mL of ethyl acetate. The organic phase was evaporated to dryness by nitrogen at 40℃ water bath. The residue was redissolved in mobile phase and then was determined. Aceclofenac was detected on Hypersil C18 column （250 mm × 4.6 mm, 5 μm） at the wavelength of 276 nm and room temperature. The mobile phase was methanol-0.1% glacial acetic acid （65：35, V/V） and the flow rate was 1.2 mL/min. Results The linear range of calibration curve was 0.1 - 15.0μg/mL with the determination limit of 0.1μg/mL. The method recoveries of low, medium and high concentration were 103.23%, 104.33%, 101.11%, respectively. The within-day and between-day RSDs were 2.800%-6.841% and 4.7020%- 8.161%, respectively. Conclusion The developed methodwith good specificity and reproducibility is easy to operate, so it is suitable for the determination of aceclofenac in plasma.

关 键 词：醋氯芬酸 血浆 高效液相色谱 

分 类 号：R927.2[医药卫生—药学]