单克隆抗体P63、SMA、PCNA在乳腺良恶性病变鉴别诊断中的意义  

Expression and significance of P63, SMA and PCNA protein in breast benign and malignant lesions

作　　者：贺海燕[1] 李永红[1] 张淼[1] 李晶[1] 

机构地区：[1]宁夏自治区人民医院病理科,宁夏银川750021

出　　处：《世界肿瘤杂志》2007年第3期162-164,共3页Tumour Journal of the World

摘　　要：目的探讨单克隆抗体P63、SMA、PCNA在乳腺疑难病变鉴别诊断中的价值。方法收集各种乳腺病变组织标本76例，采用免疫组织化学染色方法，同时标记P63、SMA、PCNA作对照研究。结果11例乳腺良性病变和12例导管原位癌中存留的肌上皮细胞几乎全部P63和SMA（＋），2例良性病变中的少数导管见P63不均匀的间断表达，P63与间质肌纤维母细胞和血管平滑肌细胞无交叉反应；在浸润性癌中仅2例见小灶状癌细胞P63（＋）。SMA除标记肌上皮细胞，还与间质肌纤维母细胞和血管平滑肌细胞有交叉反应。PCNA在浸润性导管癌阳性率92．7％，在良性病变中阳性率72．7％。结论P63可作为识别乳腺肌上皮细胞的一个新的特异性标志，同时检测P63、SMA有助于提高乳腺疾病的诊断准确率。PCNA表达与否在乳腺良恶性病变鉴别诊断中意义有限。Objective To investigate P63, SMA and PCNA protein and its signficance in breast lesions with difficulties in pathology diagnosis. Methods We compared the patterns of reactivity of antibodies with P63, SMA and PCNA on breast lesions by immunohistochemical SP method. Results Tumor cells in 2 cases showed focal P63 expression. 2 cases of benign lesions showed focal gaps in the myoepithelial cell layer, and no case showed P63 expression by myofibroblasts or vascular smooth muscle cells with difficulties in pathology diagnosis. Positive rate of PCNA expression in infiltrative ductal carcinoma was 92.7%, and 72.7% in benign breast disease. Conclusions P63 may represent a new myoepithelial marker, and the combined detection for P63 and SMA may increase the diagnostic accuracy of breast lesions. The role of PCNA expression in differential diagnosis from breast benign and malignant lesions is limited.

关 键 词：单克隆抗体 P63 SMA PCNA 乳腺疾病 

分 类 号：R655.8[医药卫生—外科学]