Induction of Cytotoxic T Lymphocytes Specific to Malignant Glioma Using T2 Cells Pulsed with HLA-A2-restricted Interleukin-13 Receptor ~2 Peptide in vitro  

作　　者：Xiaobing JIANG Xiaoling LU Ru'en LIU Fangcheng ZHANG Hongyang ZHAO 

机构地区：[1]Department of Neurosurgery Union Hospital, Tongji Medical College, Huazhong Universiry, of Science and Technology, Wuhan 430022, China [2]Department of Immunology, Shanghai Medical College, Fudan Universi~, Shanghai 200032, China [3]Department of Neurosurgery, China-Japan Friendship Hospital, Beijing 100029, China

出　　处：《Acta Biochimica et Biophysica Sinica》2007年第9期641-648,共8页生物化学与生物物理学报（英文版）

基　　金：This work was supported by a grant from the National Natural Science Foundation of China （No. 30500522）Acknowledgements We thank Dr. Wenhua WU and Dr. Guo＇an CHEN in the Wuhan Blood Center （Wuhan, China） for their kind assistance with this work.

摘　　要：Interleukin-13 receptor α2 （IL-13Rα2） is a glioma-restricted cell-surface epitope not otherwise detected within the central nervous system. The present study is a report of a novel approach of targeting malignant glioma with IL-1 3Rα2-specific cytotoxic T lymphocyte （CTL） induced from the peripheral blood mononuclear cells of healthy donors by multiple stimulations with human leukocyte antigen （HLA）-A2-restricted IL-1 3Rα2345-353 peptide-pulsed T2 cells. The induced CTL showed specific lysis against T2 cells pulsed with the peptide and HLA-A2^＋ glioma cells expressing IL- 1 3Rα2345-353, while HLA-A2 glioma cell lines that express IL-13Rα2345-353 could not be recognized by CTL. The peptide-specific activity was inhibited by anti-HLA class I monoclonal antibody. These results suggest that the induced CTL specific for IL-1 3Rα2345-353 peptide could be a potential target of specific immunotherapy for HLA-A2 patients with malignant glioma.Interleukin-13 receptor α2 （IL-13Rα2） is a glioma-restricted cell-surface epitope not otherwise detected within the central nervous system. The present study is a report of a novel approach of targeting malignant glioma with IL-1 3Rα2-specific cytotoxic T lymphocyte （CTL） induced from the peripheral blood mononuclear cells of healthy donors by multiple stimulations with human leukocyte antigen （HLA）-A2-restricted IL-1 3Rα2345-353 peptide-pulsed T2 cells. The induced CTL showed specific lysis against T2 cells pulsed with the peptide and HLA-A2^＋ glioma cells expressing IL- 1 3Rα2345-353, while HLA-A2 glioma cell lines that express IL-13Rα2345-353 could not be recognized by CTL. The peptide-specific activity was inhibited by anti-HLA class I monoclonal antibody. These results suggest that the induced CTL specific for IL-1 3Rα2345-353 peptide could be a potential target of specific immunotherapy for HLA-A2 patients with malignant glioma.

关 键 词：malignant glioma interleukin-13 receptor α2 human leukocyte antigen-A2 cytotoxic T lymphocyte 

分 类 号：Q5[生物学—生物化学]