黄酮类化合物的提取·分离纯化和含量测定方法的研究进展  

Research Advances in Extraction, Isolation, Purification and Content Determination of Methods Flavonoids

作　　者：郭雪峰[1] 岳永德[2] 

机构地区：[1]国际竹藤网络中心 [2]国家林业局竹藤科学与技术重点开放实验室,北京100102

出　　处：《安徽农业科学》2007年第26期8083-8086,共4页Journal of Anhui Agricultural Sciences

基　　金：国家林业局"948"项目资助(98006);国家"十一五"科技支撑项目(2006BAD24B0802)。

摘　　要：综述了黄酮类化合物提取方法(溶剂法、微波法、酶解法、超临界流体萃取法、双水相萃取法、超声波提取法),分离纯化方法(柱层析法、薄层层析法、高效液相色谱法、纸层析法、超临界流体分离法、大孔树脂吸附法、液滴逆流层析法、梯度pH值萃取法、金属试剂络合沉淀法、膜分离法、活性炭吸附法),黄酮含量测定方法(分光光度法、高效液相色谱法、毛细管电泳法、超临界流体色谱法、薄层扫描法),为黄酮类化合物研究工作者提供参考。Extraction methods for flavonoids （solvent method, microwave method, enzyme decomposition method, supercritical fluid extraction method, aqueous two phase extraction and ultrasonic extraction method） were summarized, as well as isolation-purification method for flavonoids （column chromatography method, thin layer chromatography method, high-performance liquid chromatography method, paper chromatography method, supercritical fluid chromatography method,macroporous resin adsorption method, high-speed counter current chromatography method, pH grads extraction method, complexation precipitate method, film filtration method and active carbon adsorption method） and determination methods for flavonoids content （spectrophotometric method, high-performance liquid chromatography method, capillary electrophoreses method, supercritical fluid chromatography method, thin layer chromatography method）. All these provided systemic reference for researchers studied on flavonoids.

关 键 词：黄酮类化合物 提取 分离 纯化 测定 

分 类 号：Q946[生物学—植物学]