大麦干种子DNA提取研究  

Study on DNA Extraction in Dry Barley Seed

作　　者：王芳[1] 

机构地区：[1]青海省农林科学院,青海西宁810016

出　　处：《安徽农业科学》2007年第26期8132-8133,共2页Journal of Anhui Agricultural Sciences

摘　　要：进行了应用改进后CTAB方法从半粒大麦种子中提取DNA的研究。结果表明,从干种子中提取的DNA是完整的,多糖等大分子及RNA也被去除;提取的DNA与大麦叶片中提取的DNA是一致的;其PCR扩增产物与从叶片中提取的DNA扩增产物有相同的条带类型。DNA was extracted from barley half seed using modified CTAB method.Result showed that the DNA extracted from dry seed was complete, which could effectively remove RNA and macromolecular. It showed the same banding patterns with those from leaf DNA. Amplification products of PCR from leaves had the same brand.

关 键 词：半粒种子 DNA提取 CTAB法 分子标记 

分 类 号：Q78[生物学—分子生物学]