油青四九菜心细胞核不育系转育研究  

Study on Transformation Breeding of Cell Nuclear Sterile Line in Youqingsijiu Brassica campestris

作　　者：赵建锋[1] 沈向群[2] 秦进华[1] 孙玉东[1] 朱明超[1] 刘友华[1] 

机构地区：[1]江苏省徐淮地区淮阴农业科学研究所,江苏淮阴223001 [2]沈阳农业大学园艺学院,辽宁沈阳223001

出　　处：《安徽农业科学》2007年第26期8141-8142,8144,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]探索油青四九菜心细胞核不育系的转育。[方法]以法45抗热大白菜不育系为不育源,通过常规杂交转育得到油青四九菜心细胞核不育系、新甲型两用系和临时保持系。[结果]用可育品系与雄性不育系中不育株和甲型两用系中不育株杂交,以及新甲型两用系中可育株正反交,测定15个菜心可育品系基因型均为MsfMsf。按雄性不育系杂交转育方案,育成了大白菜细胞质油青四九菜心的新甲型两用系,雄性不育系和临时保持系。按照回交转育方案,完成了大白菜细胞质和菜心细胞质4个世代雄性不育系的转育,其后代雄蕊育性分离比例完全符合回交转育模式。[结论]在雄性不育系的转育过程中,注意同型株的选择,会有很好的转育效果。[Objective] The aim of the research was to discuss transformation breeding of cell nuclear sterile line in Youqingsijiu Brassica campestris. [Method] Taking French 45 hot-resistance Chinese cabbage sterile line as sterile source, cell nuclear sterile line and new AB lines type I and temporary maintainer line of Youqingsijiu B. campestris were obtained through transformation breeding of conventional hybrid. [Result] Based on the cross between the fertile lines and the sterile plants in male sterile lines and that in AB lines type I and reciprocal cross between fertile plants in new AB lines type I, the genotypes of fertile lines were all determined as MsfMsf. According to transformation breeding project of male sterile line hybrid, new AB lines type I, male sterile line and temporary maintainer line of Youqingsijiu B. campestris with Chinese cabbage cytoplasm were bred. According to backcross transformation breeding project, transformation breeding of 4 generation male sterile lines with cytoplasm of Chinese cabbage and B. campestris were completed, whose separation proportion of stamen fertility progenies was accorded with backcross transformation breeding project completely. [Conclusion] During the transformation breeding of male sterile line, it would had good effect of transformation breeding to pay attention to select the plants with the same type,

关 键 词：油青四九菜心 细胞核不育系 大白菜 

分 类 号：S565.4[农业科学—作物学]