不同猪苓菌核分离营养菌丝的研究  

Research on Separating Vegetative Hyphae from Different Polyporus umbellate Sclerotia

作　　者：陈永刚 邓百万[2] 陈文强[2] 彭浩[2] 刘开辉[2] 魏巍[2] 刘飞[2] 

机构地区：[1]陕西东盛生物制品有限公司,陕西汉中723000 [2]陕西理工学院生物科学与工程学院,陕西汉中723001

出　　处：《安徽农业科学》2007年第28期8840-8841,共2页Journal of Anhui Agricultural Sciences

基　　金：陕西教育厅重点实验室重点科研项目(04JS32)

摘　　要：[目的]为了明确猪苓的种质资源,为其大面积栽培提供理论依据。[方法]采用组织培养法,分离秦巴山区3种猪苓营养菌丝。[结果]结果表明,在温度25~28℃、pH值自然的条件下,用CPDA培养基和蛋白胨培养基培养,不同猪苓菌核均能分离出营养菌丝。[结论]综合不同猪苓菌核分离营养菌丝的萌发时间和萌发率、菌丝密度和生长势等因素,发现CPDA培养基较适合作为分离培养基。[Objective] The aim was to confirm the germplasm resources so as to provide theoretical foundation for its cultivation in large area.[Method] The vegetative hypha of 3 different varieties of Polyporus umbellate from Qinba mountainous areas were separated by tissue culture method.[Result] Under the temperature of 25～28 ℃ and the natural pH condition,the vegetative hypha could be separated from different P.umbellate sclerotia by using CPDA medium and peptone medium.[Conclusion] Integrating the factors such as germination time,germination rate,hyphae density and growth of the vegetative hyphae separated from different P.umbellate sclerotia,CPDA medium was more suitable to be as the separating medium.

关 键 词：猪苓 菌核 分离 营养菌丝 

分 类 号：S646[农业科学—蔬菜学]