链霉素抗性筛选井冈霉素高产菌株的研究  

Study on the Streptomycin Resistance Screening of High-yield Strain of Jinggangmycin

作　　者：牛莉娜 葛翠凤[1] 葛绍荣[1] 

机构地区：[1]四川大学生物资源与生态环境教育部重点实验室,四川成都610064

出　　处：《安徽农业科学》2007年第28期8930-8932,共3页Journal of Anhui Agricultural Sciences

摘　　要：对井冈霉素产生菌吸水链霉菌井冈变种SJ-6(Streptomyce shygroscopicusvar. Jingganggensis Yen)进行UV和亚硝酸复合诱变,在含有菌株孢子最小抑制浓度的链霉素培养基平板上,采用链霉素抗性筛选法获得大量的链霉素抗性突变株,筛选到突变株UN-80,在摇瓶发酵条件下,井冈霉素化学效价达15 943μg/ml,比出发菌株SJ-6提高了84.4%。该菌株连续培养5代,其化学效价基本稳定,具有较好的遗传稳定性。UV and nitrous acid complex mutagenesis was conducted on Streptomyces hygroscopicus var.Jingganggensis Yen SJ-6 produced Jinggangmycin.In the medium plate with streptomycin at minimum inhibiting concentration of strain spores,a lot of streptomycin resistant mutants were obtained by streptomycin resistance screening,including the mutant UN-80.Under the condition of shaking flask fermentation,the Jinggangmycin chemical titer reached 15 943 μg/ml,increased 84.4% than the original strain SJ-6.After continuously cultured for 5 generations the strain had basically stable chemical titer and better hereditary stability.

关 键 词：链霉素抗性筛选 UV诱变 亚硝酸诱变 井冈霉素 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]