端粒酶反义寡核苷酸对肝癌细胞生长抑制作用  

Effect of Telomerase Antisense Oligonucleotides on Inhibiting Growth of Hepatocarcinoma Cell

作　　者：张英哲[1] 许德顺[1] 单伟[1] 孙大鹏[1] 任延鹏[1] 

机构地区：[1]辽宁医学院,辽宁锦州121001

出　　处：《辽宁医学院学报》2007年第4期18-20,共3页Journal of Liaoning Medical University (LNMU) Bimonthly

摘　　要：目的本研究将互补于hTR模板区的全硫代修饰型反义寡核苷酸(PS-ASODN)经lipotap脂质体转染作用于肝癌细胞系BEL-7402,观察其对肝癌细胞端粒酶活性的影响,同时检测其对癌细胞的生长抑制作用,旨在探讨PS-ASODN对肝癌的治疗价值,为临床治疗肝癌提供一定的试验数据。方法分别采用MTT法、流式细胞术、酶联免疫吸附(ELISA)法检测不同浓度PS-ASODN对bel-7402的细胞增殖、细胞凋亡和端粒酶活性的作用。结果PS-ASODN能抑制BEL-7402细胞的生长,降低其端粒酶活性并诱导细胞凋亡。结论端粒酶与BEL-7402增殖活性有关,抑制端粒酶活性可能成为肝癌基因治疗的新靶点。Objective The research would complement PS-ASODN in hTR which was affected by lipotap and had effect on Hepatocarcinoma cell BEL-7402,investigating the effect of phosphorothioate antisense oligonucleotide（PS-ASODN） on telomerase activity changes of liver cancer cell and testing the effect on inhabiting the growth of liver cancer as well as exploring the treatment value of PS-ASODN for liver cancer.It provided some experimental data for clinical treatment for liver cancer. Methods The effect of different density of PS-ASODN on proliferation activity of cells,cells＇withering,activity of Telomerase of bel-7402 were examined by methyl thiazolyl tetrazolium（MTT） flow cytometer method and ELISA. Results Growth of BEL-7402 Cell were inhibited by PS-ASODN and telomerase＇activity was decreased by it,also causing the revulsion of cells＇death. Conclusions The results suggested that telomerase was related to BEL-7402 proliferation activity.Inhibition of telomerase activity would be a new target to treatment for hepatocarcinoma.

关 键 词：端粒酶 肝癌细胞 全硫代反义寡核苷酸 

分 类 号：Q74[生物学—分子生物学]