孕期接触褪黑素对细菌脂多糖引起的小鼠炎性细胞因子释放的影响  

Maternally Administered Melatonin Differentially Regulates Lipopolysaccharide-induced Inflammatory Cytokines in Mice

作　　者：王华[1] 徐德祥[1] 宁萑[1] 吕金伟[1] 赵磊[1] 李祥云[1] 张程[1] 

机构地区：[1]安徽医科大学卫生毒理学系,合肥230032

出　　处：《生殖与避孕》2007年第9期573-576,共4页Reproduction and Contraception

基　　金：国家自然科学基金(30371667;30572223;30671786);校基金(编号:2006kj13)

摘　　要：目的:研究孕期接触褪黑素(MT)对细菌脂多糖(LPS)引起小鼠炎性细胞因子释放的影响。方法:孕17d鼠被随机分为对照组、LPS组和MT+LPS组,每组12只。LPS组、MT+LPS组孕鼠均给予LPS(500μg/kg,i.p.);MT+LPS组在LPS处理前0.5h给予MT(5mg/kg,i.p.);对照组孕鼠给予等容积生理盐水。于LPS或生理盐水处理1.5h后摘眼球取血,处死孕鼠,并留取羊水、胎肝、胎脑。用ELISA法分别测定母血、羊水、胎肝和胎脑中TNF-α、IL-1β、IL-6及IL-10含量。结果:LPS能显著升高母血、羊水和胎肝中TNF-α、IL-1β、IL-6及IL-10含量,MT+LPS组母血和胎肝中IL-10含量明显高于LPS组,MT预处理显著抑制LPS引起的母血TNF-α释放,但MT对LPS引起的母血和羊水中IL-1β、IL-6含量的变化无明显影响。此外,LPS显著升高胎脑中TNF-α和IL-10含量,而MT预处理却明显降低LPS引起的胎脑TNF-α释放。结论:孕期接触MT可多向调节LPS诱发的小鼠母血、羊水、胎肝和胎脑中促炎细胞因子与抗炎细胞因子释放。Objective： To investigate the effects of melatonin on LPS-evoked releases of cytokines in maternal serum, anmiotic fluid, fetal liver and fetal brain. Methods： The d 17 of pregnant mice were divided randomly into three groups and 12 mice in each group. All pregnant mice except controls （saline） received an intraperitoneal （i.p.） injection of LPS （500 μg/kg）. In MT＋LPS group, pregnant mice were treated with 5 mg/kg MT （i.p.） at 30 rain before LPS. All mice were sacrificed at 1.5 h after LPS. Blood serum and amniotic fluid were collected for measurements of TNF-α, IL-1β, IL-6, IL-10 by ELISA. Fetal Livers and fetal brains were dissected for measurements of TNF-α, IL-1β, IL-6, IL-10 by ELISA. Results： Maternal LPS exposure increased the levels ofTNF-α, IL-1β and IL-6 in fetal liver and fetal brain. Melatonin pretreatment effectually inhibited LPS-evoked proinflammatory cytokine TNF-α in maternal serum, amniotic fluid and fetal brain. On the other hand, this immunomodulator augmented the release of antiinflammtory cytokine IL- 10 in maternal serum and fetal liver of LPS- treated mice. Conclusion： Maternally adminstered melatonin differentially regulates LPS-induced proinflammatory and antiinflammatory cytokines in maternal serum, amniotic fluid, fetal liver and fetal brain.

关 键 词：褪黑素(MT) 细菌脂多糖(LPS) 细胞因子 胎儿 羊水 

分 类 号：R714.2[医药卫生—妇产科学]