COL4A1基因多态性与中国女性骨密度关系分析  

The relationship between polymorphism of COL4A1 gene and bone mineral density in Chinese women

作　　者：吴涤[1] 洪秀梅[1] 蒋英俊[1] 李志平[2] 臧桐华[1] 

机构地区：[1]安徽医科大学安徽省生物医学研究所,合肥230032 [2]安徽医科大学生物医学研究所安庆中心

出　　处：《中国骨质疏松杂志》2007年第1期42-44,58,共4页Chinese Journal of Osteoporosis

基　　金：安徽省自然科学基金资助项目(01043802)

摘　　要：目的 研究COL 4 A 1基因多态性与中国农村女性骨密度关系.方法 采用双能X线吸收仪对343名女性研究对象进行腰椎及股骨扫描,应用PCR-限制性片段长度多态性（PCR-RFLP）方法检测COL4 A 1基因（谷酰氨酸Q 1334 H组氨酸）多态位点基因型,用广义线性模型分析此位点多态性与腰椎2～4及股骨骨密度关系.结果 在调整环境危险因素后,均未显示COL 4 A 1基因H 1334 H基因型与股骨颈、腰椎2～4骨密度相关（P=0.409、0.705）.结论 在中国农村女性人群中COL 4 A 1基因Q 1334 H多态性与股骨颈、腰椎2～4骨密度无显著相关性.Objective To investigate the association of polymorphism of collagen type Ⅳ alpha 1 gene （ COL 4 A 1 ） with Bone Mineral Density （BMD） in rural Chinese women. Methods The bone mineral density was measured at the lumbar spine and femur using dual-energy X-ray absorptiometry in 343 study subjects. PCR-restriction fragment length polymorphism （PCR-RFLP） was applied to examine Q 1334 H polymorphism of the COL 4 A 1 gene. Generalized linear model was used to analysize the association of this polymorphism with the bone mineral density of the lumbar spine （ L2-4 ） and femur. Results After adjustment for environmental covariates, no significant correlation was found between the Q 1334 H polymorphism of the COL 4 A 1 gene and BMD, including femoral neck, lumbar spine （ L2-4 ） （ P = 0.409,0.705 respectively ）. Conclusion There is no significant relationship between the Q 1334 H polymorphism of the COL 4 A 1 gene and BMD of femoral neck and lumbar spine （2-4） in rural Chinese women population.

关 键 词：Ⅳ型胶原α链(COL 4 A 1) 多态性 相关性 骨矿物质密度(BMD) 

分 类 号：R541.4[医药卫生—心血管疾病]